Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines

Version 1 : Received: 1 June 2021 / Approved: 2 June 2021 / Online: 2 June 2021 (07:21:59 CEST)

A peer-reviewed article of this Preprint also exists.

Kfoury, M.; Mouawad, C.; Rifi, M.; Sadek, R.; Sabatier, J.-M.; Nehme, H.; Fajloun, Z. Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines. Sci 2021, 3, 31. Kfoury, M.; Mouawad, C.; Rifi, M.; Sadek, R.; Sabatier, J.-M.; Nehme, H.; Fajloun, Z. Montivipera bornmuelleri Venom: Inhibitory Effect on Staphylococcus epidermidis and Escherichia coli F1F0-ATPases and Cytotoxicity on HCT116 Cancer Cell Lines. Sci 2021, 3, 31.

Journal reference: Sci 2021, 3, 31
DOI: 10.3390/sci3030031

Abstract

In this work, we pursued the biological characterization of the venom of Montivipera born-muelleri, a viper from the Lebanese mountains. In relativity to its antibacterial potential, the in-hibitory effect of this venom on the F1F0-ATPase enzymes of Gram-positive Staphylocoocus epider-midis and Gram-negative Escherichia coli bacteria was examined. In order to determine the de-gree of cytotoxicity of the venom on the HCT116 human colon cancer cell lines, the biological MTT proliferation and cell viability test were implemented. After validation of the enzymatic F1F0-ATPase model by the spectrophotometric method, using quercetin as the reference ligand, re-sults revealed that M. bornmuelleri venom is able to inhibit the activity of the enzyme of these two bacteria with a concentration of the order of 100-150 µg/mL. In addition, a venom concentration of 10 µg/mL was sufficient to kill the totality of HCT116 cell lines cultivated in vitro. These data show that M. bornmuelleri venom is a mixture of diverse molecules presenting activities of interest and is a potential source to explore in order to discover new drug candidates.

Keywords

Montivipera bornmuelleri snake venom; F1F0-ATPase; Staphylococcus epidermidis; Escherichia coli; antibacterial activity; HCT116 cells; anticancer activity

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