Gold-conjugated Nanobodies for Targeted Imaging using High-resolution Secondary Ion Mass Spectrometry

Paola Agüi-Gonzalez; Tal M. Dankovich; Felipe Opazo; Silvio O. Rizzoli; Nhu T.N. Phan

doi:10.20944/preprints202105.0687.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 27 May 2021 / Approved: 28 May 2021 / Online: 28 May 2021 (09:57:12 CEST)

Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in secondary ion mass spectrometry (SIMS) of biological samples. This is because only a few isotopic tags can be used successfully to target specific proteins or organelles. To address this, we have generated gold-nanoprobes, in which gold nanoparticles are conjugated to nanobodies. The nanoprobes were well suited for specific molecular imaging using NanoSIMS at subcellular resolution. They demonstrated to be highly selective to different proteins of interest, and sufficiently sensitive for SIMS detection. The nanoprobes offer the possibility of correlating the investigation of cellular isotopic turnover to the positions of specific proteins and organelles, thereby enabling an understanding of functional and structural relations that are currently obscure.

nanobodies; gold nanoparticles; target imaging; nanoSIMS; SIMS

CHEMISTRY, Analytical Chemistry

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Gold-conjugated Nanobodies for Targeted Imaging using High-resolution Secondary Ion Mass Spectrometry

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