Article
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Preserved in Portico This version is not peer-reviewed
Gold-conjugated Nanobodies for Targeted Imaging using High-resolution Secondary Ion Mass Spectrometry
Version 1
: Received: 27 May 2021 / Approved: 28 May 2021 / Online: 28 May 2021 (09:57:12 CEST)
A peer-reviewed article of this Preprint also exists.
Journal reference: Nanomaterials 2021, 11, 1-12
DOI: 10.3390/nano11071797
Abstract
Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in secondary ion mass spectrometry (SIMS) of biological samples. This is because only a few isotopic tags can be used successfully to target specific proteins or organelles. To address this, we have generated gold-nanoprobes, in which gold nanoparticles are conjugated to nanobodies. The nanoprobes were well suited for specific molecular imaging using NanoSIMS at subcellular resolution. They demonstrated to be highly selective to different proteins of interest, and sufficiently sensitive for SIMS detection. The nanoprobes offer the possibility of correlating the investigation of cellular isotopic turnover to the positions of specific proteins and organelles, thereby enabling an understanding of functional and structural relations that are currently obscure.
Keywords
nanobodies; gold nanoparticles; target imaging; nanoSIMS; SIMS
Subject
CHEMISTRY, Analytical Chemistry
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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