Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Designing a Multi-Epitope Vaccine against Dracunculus medinensis by Employing Immuno-Informatics and In Silico Approaches

Version 1 : Received: 16 May 2021 / Approved: 18 May 2021 / Online: 18 May 2021 (09:31:15 CEST)

How to cite: Sarfraz, M.T.; Rana, M.M. Designing a Multi-Epitope Vaccine against Dracunculus medinensis by Employing Immuno-Informatics and In Silico Approaches. Preprints 2021, 2021050400 (doi: 10.20944/preprints202105.0400.v1). Sarfraz, M.T.; Rana, M.M. Designing a Multi-Epitope Vaccine against Dracunculus medinensis by Employing Immuno-Informatics and In Silico Approaches. Preprints 2021, 2021050400 (doi: 10.20944/preprints202105.0400.v1).

Abstract

Dracunculiasis (also known as Guinea worm disease) is caused by Dracunculus medinensis parasite and it spreads by drinking water containing Larvae of Guinea worm. The lack of safe water facilities, preventions and treatments resulted in highly dangerous consequences in its endemic regions. The economy of the affected regions totally falls down due to less production which is the result of agricultural field worker’s bad health. In this study, a multi epitope vaccine was designed against Dracunculus medinensis by using immune-informatics. The vaccine was designed by using T-Cell and B-Cell epitopes derived from Dracunculus medinensis proteins (Lactamase-B domain-containing protein, G-Domain containing protein and Ferrochelatase) in addition to Adjuvants and Linkers. The tertiary structure, physiochemical properties and immunogenic elements of vaccine were achieved. The validation of tertiary structure was accessed, and quality was achieved. In addition, the world coverage of parasite’s CTL and HTL epitopes is 95.61%. The stability of the chimeric vaccine was achieved through disulfide engineering. The molecular docking with Toll Like Receptor 4 (TLR-4) of vaccine showed its binding efficiency followed by Molecular Dynamic Simulation. The immune simulation suggested the mediated cell immunity and repeated antigen clearance. At the end, the optimized codon was used in in silico cloning to ensure vaccine’s higher exposure in bacterium E. coli strain K12. With further assessments, it is believed that the proposed multi epitope vaccine has strong immunogen to control Dracunculus medinensis which may result in better social and economic conditions of endemic regions.

Subject Areas

Immuno-informatics; Multi-epitope; Dracunculiasis

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