Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fluorescence Anisotropy Studies of Fluorescein

Version 1 : Received: 5 May 2021 / Approved: 10 May 2021 / Online: 10 May 2021 (13:41:06 CEST)

How to cite: Jain, P.; Motosuke, M.; Aida, T. Fluorescence Anisotropy Studies of Fluorescein. Preprints 2021, 2021050189 (doi: 10.20944/preprints202105.0189.v1). Jain, P.; Motosuke, M.; Aida, T. Fluorescence Anisotropy Studies of Fluorescein. Preprints 2021, 2021050189 (doi: 10.20944/preprints202105.0189.v1).

Abstract

Fluorescein anisotropy, which is a widely used technique to study the folding state of proteins or affinity of ligands, is used in the present work to study the temperature sensing of fluid in a microchannel, by adding fluorophore in the fluid. Fluorescein was used as a temperature probe, while glycerol-aq. ammonia was used as a working fluid. Fluorescence anisotropy of fluorescein were measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed.

Subject Areas

Fluorescence Anisotropy; Fluorescein; Glycerol

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