Fluorescence Anisotropy Studies of Fluorescein

Puneet Jain; Masahiro Motosuke; Takuya Aida

doi:10.20944/preprints202105.0189.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 5 May 2021 / Approved: 10 May 2021 / Online: 10 May 2021 (13:41:06 CEST)

Fluorescein anisotropy, which is a widely used technique to study the folding state of proteins or affinity of ligands, is used in the present work to study the temperature sensing of fluid in a microchannel, by adding fluorophore in the fluid. Fluorescein was used as a temperature probe, while glycerol-aq. ammonia was used as a working fluid. Fluorescence anisotropy of fluorescein were measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed.

Fluorescence Anisotropy; Fluorescein; Glycerol

Chemistry and Materials Science, Biomaterials

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Fluorescence Anisotropy Studies of Fluorescein

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