Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Detection of Fecal SARS-CoV-2 RNA in a Prospective Cohort of Children with Multisystem Inflammatory Syndrome (MIS-C)

Version 1 : Received: 9 May 2021 / Approved: 10 May 2021 / Online: 10 May 2021 (13:00:43 CEST)

How to cite: Parodi, E.; Carpino, A.; Franchitti, E.; Pruccoli, G.; Denina, M.; Pagliero, F.; Ramenghi, U.; Traversi, D. Detection of Fecal SARS-CoV-2 RNA in a Prospective Cohort of Children with Multisystem Inflammatory Syndrome (MIS-C). Preprints 2021, 2021050187 (doi: 10.20944/preprints202105.0187.v1). Parodi, E.; Carpino, A.; Franchitti, E.; Pruccoli, G.; Denina, M.; Pagliero, F.; Ramenghi, U.; Traversi, D. Detection of Fecal SARS-CoV-2 RNA in a Prospective Cohort of Children with Multisystem Inflammatory Syndrome (MIS-C). Preprints 2021, 2021050187 (doi: 10.20944/preprints202105.0187.v1).

Abstract

Multisystem inflammatory syndrome related to SARS-CoV-2 infection (MIS-C) is a rare severe illness affecting pediatric patients. No data are available in literature about the presence of SARS-CoV-2 RNA in faeces in patients with MIS-C. The SARS-CoV-2 positivity was evaluated in fecal samples obtained in a prospective cohort of pediatric patients admitted to our tertiary Hos-pital and diagnosed with MIS-C or COVID-19 between April 15, 2020, and February 29, 2021. The real-time RT-PCR was performed using a validated kit. The assay measures 3 target genes: SARS-CoV-2 gene E, gene N, gene ORF1ab. Overall, 16/63 (25%) fecal samples revealed the pres-ence of SARS -CoV-2 RNA. No differences were detected about time from presumptive viral ex-posure and the time of stool collection (14 vs 8 days, p> 0,05) as well as about the presence of gas-trointestinal symptoms (p>0.05) between patients with positive (+) stools for SARS-CoV-2 RNA and patients tested negative (-). Among children with MIS-C, stools were collected 27.5 days as median (95% CI 14-34) after presumed contact and the positivity rate was 12.5% (4/32). According to our data, we could suggest contact precautions with all patients with MIS-C. Further sample collection is ongoing to verify our preliminary data.

Keywords

SARS-CoV-2; stools; COVID-19; MIS-C; children

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