Working Paper Article Version 1 This version is not peer-reviewed

Photothermal Killing of A549 Cells and Autophagy Induction by Bismuth Selenide Nanoparticles

Version 1 : Received: 9 May 2021 / Approved: 10 May 2021 / Online: 10 May 2021 (10:52:30 CEST)

A peer-reviewed article of this Preprint also exists.

You, Y.; Li, J.; Chen, L.; Wang, M.; Dong, X.; Yan, L.; Zhang, A.; Zhao, F. Photothermal Killing of A549 Cells and Autophagy Induction by Bismuth Selenide Particles. Materials 2021, 14, 3373. You, Y.; Li, J.; Chen, L.; Wang, M.; Dong, X.; Yan, L.; Zhang, A.; Zhao, F. Photothermal Killing of A549 Cells and Autophagy Induction by Bismuth Selenide Particles. Materials 2021, 14, 3373.

Journal reference: Materials 2021, 14, 3373
DOI: 10.3390/ma14123373

Abstract

With a highly efficient optical absorption capability, bismuth selenide (Bi2Se3) nanomaterial can be used as an outstanding photothermal agent for anti-tumor treatment and shows promise in the field of nanotechnology-based biomedicine. However, little research has been done on the relevant mechanism underlying the photothermal killing effect of Bi2Se3 nanomaterial. Herein, the photothermal effects of Bi2Se3 nanoparticles on A549 cells were explored with emphasis put on autophagy. Firstly, we characterized the structure and physicochemical property of the synthesized Bi2Se3 and confirmed their excellent photothermal conversion efficiency (35.72%), photostability, biocompatibility and ability of photothermal killing on A549 cells. Enhanced autophagy was detected in Bi2Se3-exposed cells under an 808 nm laser. Consistently, an elevated expression ratio of LC3-II to LC3-I, a marker of autophagy occurrence, was induced in Bi2Se3-exposed cells upon NIR irradiation. Meanwhile, the expression of cleaved-PARP was increased in the irradiated cells dependently on the exposure concentrations of Bi2Se3 nanoparticles. Pharmacological inhibition of autophagy by 3-methyladenine (3-MA) further strengthened the photothermal killing effect of Bi2Se3. Meanwhile, stress-related signaling pathways including p38 and SAPK/JNK were activated coupled with the attenuated PI3K/Akt signaling. Our study figures out that autophagy and the activation of stress-related signaling pathways were involved in the photothermal killing of cancerous cells by Bi2Se3, which provides a more understanding of photothermal nanomaterials.

Subject Areas

bismuth selenide nanoparticles; photothermal killing; apoptosis; autophagy; stress-related signaling pathway

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