Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Pitfalls and Caveats in Applying Immunostaining to Histopathological Diagnosis

Version 1 : Received: 27 April 2021 / Approved: 28 April 2021 / Online: 28 April 2021 (14:17:11 CEST)

A peer-reviewed article of this Preprint also exists.

Tsutsumi, Y. Pitfalls and Caveats in Applying Chromogenic Immunostaining to Histopathological Diagnosis. Cells 2021, 10, 1501. Tsutsumi, Y. Pitfalls and Caveats in Applying Chromogenic Immunostaining to Histopathological Diagnosis. Cells 2021, 10, 1501.

Journal reference: Cells 2021, 10, 1501
DOI: 10.3390/cells10061501

Abstract

Immunostaining is an essential histochemical technique for analyzing pathogenesis and making a histopathological diagnosis. The needs are prompted by technical development and refinement, commercial availability of a variety of antibodies, deepened knowledge of immunohistochemical markers, accelerated analysis of morphofunctional correlations, progress in molecular target therapy, and the expectation of advanced histopathological diagnosis. However, immunostaining does have various pitfalls and caveats. We should learn from mistakes and failures, as well as from false positivity and false negativity. The present review article describes various devices, technical hints and trouble-shooting guides to keep in mind in performing immunostaining.

Keywords

Epitope retrieval; Fixation; Histopathological diagnosis; Immunostaining; Sensitivity; Specificity; Trouble-shooting

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