Review
Version 1
Preserved in Portico This version is not peer-reviewed
Pitfalls and Caveats in Applying Immunostaining to Histopathological Diagnosis
Version 1
: Received: 27 April 2021 / Approved: 28 April 2021 / Online: 28 April 2021 (14:17:11 CEST)
A peer-reviewed article of this Preprint also exists.
Tsutsumi, Y. Pitfalls and Caveats in Applying Chromogenic Immunostaining to Histopathological Diagnosis. Cells 2021, 10, 1501. Tsutsumi, Y. Pitfalls and Caveats in Applying Chromogenic Immunostaining to Histopathological Diagnosis. Cells 2021, 10, 1501.
Abstract
Immunostaining is an essential histochemical technique for analyzing pathogenesis and making a histopathological diagnosis. The needs are prompted by technical development and refinement, commercial availability of a variety of antibodies, deepened knowledge of immunohistochemical markers, accelerated analysis of morphofunctional correlations, progress in molecular target therapy, and the expectation of advanced histopathological diagnosis. However, immunostaining does have various pitfalls and caveats. We should learn from mistakes and failures, as well as from false positivity and false negativity. The present review article describes various devices, technical hints and trouble-shooting guides to keep in mind in performing immunostaining.
Keywords
Epitope retrieval; Fixation; Histopathological diagnosis; Immunostaining; Sensitivity; Specificity; Trouble-shooting
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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