Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Possible Functions of P21 CDKN1A within the Germ Cells of the Mouse Seminiferous Epithelium

Version 1 : Received: 18 April 2021 / Approved: 21 April 2021 / Online: 21 April 2021 (08:46:38 CEST)

How to cite: Pedro, A. Possible Functions of P21 CDKN1A within the Germ Cells of the Mouse Seminiferous Epithelium. Preprints 2021, 2021040558 (doi: 10.20944/preprints202104.0558.v1). Pedro, A. Possible Functions of P21 CDKN1A within the Germ Cells of the Mouse Seminiferous Epithelium. Preprints 2021, 2021040558 (doi: 10.20944/preprints202104.0558.v1).

Abstract

The cyclin dependent kinase inhibitor P21 CDKN1A has been shown to be upregulated during differentiation and after DNA damage in somatic cells. We examined the expression of P21 CDKN1A and of γ-H2AX during the differentiation of germ cells in normal mouse testis. P21 CDKN1A was normally expressed in in a variable mode in different cell types (spermatogonia, spermatocytes, spermatids, Sertoli cell) located since the basal till the lumen of the seminiferous tubules. Moreover, in agreement with γ-H2AX expression, P21 maybe involved in different cell cycle checkpoints in spermatocytes such as a pre-replication, XY body inactivation, a mid-pachytene and a metaphase I checkpoints. Finally, in comparison to control, the p21 deficient mouse testis show elevated numbers of apoptosis of elongated spermatids within stages VIII-IX but any difference in the number of spermatogonia mitosis.These results suggest that P21 also may take part in the regulation of the differentiation of the male germ cells and may have a role in spermatogonia not related with a mitotic checkpoint.

Subject Areas

P21 CDKN1A; Spermatogenesis

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.