Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11

Version 1 : Received: 16 April 2021 / Approved: 19 April 2021 / Online: 19 April 2021 (12:17:34 CEST)

A peer-reviewed article of this Preprint also exists.

Tokumoto, S.; Miyata, Y.; Deviatiiarov, R.; Yamada, T.G.; Hiki, Y.; Kozlova, O.; Yoshida, Y.; Cornette, R.; Funahashi, A.; Shagimardanova, E.; Gusev, O.; Kikawada, T. Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11. Int. J. Mol. Sci. 2021, 22, 5798. Tokumoto, S.; Miyata, Y.; Deviatiiarov, R.; Yamada, T.G.; Hiki, Y.; Kozlova, O.; Yoshida, Y.; Cornette, R.; Funahashi, A.; Shagimardanova, E.; Gusev, O.; Kikawada, T. Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11. Int. J. Mol. Sci. 2021, 22, 5798.

Journal reference: Int. J. Mol. Sci. 2021, 22, 5798
DOI: 10.3390/ijms22115798

Abstract

Pv11, an insect cell line established from the midge Polypedilum vanderplanki, is capable of ametabolic desiccation tolerance, so-called anhydrobiosis. We previously discovered that heat shock factor 1 (HSF1) contributes to the acquisition of desiccation tolerance by Pv11 cells, but the mechanistic details have yet to be elucidated. Here, by analyzing the gene expression profiles of newly established HSF1-knockout and -rescue cell lines, we show that HSF1 has a genome-wide effect on gene regulation in Pv11. HSF1-knockout cells exhibit a reduced desiccation survival rate, but this is completely restored in HSF1-rescue cells. By comparing mRNA profiles of the two cell lines, we reveal that HSF1 induces anhydrobiosis-related genes, especially genes encoding late embryogenesis abundant proteins and thioredoxins, but represses a group of genes involved in basal cellular processes, thus promoting an ametabolic state in the cell. In addition, HSF1 binding motifs are enriched in the promoters of anhydrobiosis-related genes and we demonstrate binding of HSF1 to these promoters by ChIP-qPCR. Thus, HSF1 directly regulates the transcription of anhydrobiosis-related genes and consequently plays a pivotal role in the induction of anhydrobiotic ability in Pv11 cells.

Subject Areas

CRISPR/Cas9; knockout; rescue; desiccation tolerance; anhydrobiosis; Polypedilum vanderplanki; HSF1; insect cell; Pv11

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