Working Paper Article Version 2 This version is not peer-reviewed

Acute Low-intensity Treadmill Running Induces Intestinal Glucose Transporters via GLP-2 in Mice

Version 1 : Received: 19 April 2021 / Approved: 19 April 2021 / Online: 19 April 2021 (11:52:31 CEST)
Version 2 : Received: 19 April 2021 / Approved: 20 April 2021 / Online: 20 April 2021 (11:48:02 CEST)

A peer-reviewed article of this Preprint also exists.

Aoki, K.; Suzuki, T.; Hui, F.; Nakano, T.; Yanazawa, K.; Yonamine, M.; Fujita, S.; Sugasawa, T.; Yoshida, Y.; Omi, N.; Kawakami, Y.; Takekoshi, K. Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice. Nutrients 2021, 13, 1735. Aoki, K.; Suzuki, T.; Hui, F.; Nakano, T.; Yanazawa, K.; Yonamine, M.; Fujita, S.; Sugasawa, T.; Yoshida, Y.; Omi, N.; Kawakami, Y.; Takekoshi, K. Acute Low-Intensity Treadmill Running Upregulates the Expression of Intestinal Glucose Transporters via GLP-2 in Mice. Nutrients 2021, 13, 1735.

Abstract

Exercise affects various organs. However, its effects on nutrient digestion and absorption in the intestinal tract are not well understood. A few studies have reported that exercise training in-creases the expression of carbohydrate digestion and absorption molecules. Exercise was also shown to increase the concentration of blood glucagon like peptide-2(GLP-2), which regulates carbohydrate digestion and absorption in small intestinal epithelium. Therefore, we investigated the effects of exercise on intestinal digestion and absorption molecules and the levels of GLP-2. 6-wk-old of male mice were divided into 2 groups; sedentary (SED) and low-intensity exercise (LEx). LEx mice were required to run on a treadmill (12.5 m/min, 60 min), whereas SED mice rested. All mice were euthanized 1 h after exercise or rest and plasma, jejunum, ileum, and colon were sampled. Samples were analyzed using EIA and immunoblotting. The levels of plasma GLP-2 and the expression of the GLP-2 receptor, sucrase-isomaltase (SI), and glucose transporter (GLUT2) in the jejunum were increased in LEx group. We showed that acute low-intensity exer-cise affects the intestinal carbohydrate digestion and absorption molecules via GLP-2. Our results suggest that exercise might provide new benefits to the small intestine for people with intestinal frailty.

Keywords

low intensity exercise; intestine; sodium-dependent glucose transporter; glucose transporter 2; glucagon like peptide 2

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Comments (1)

Comment 1
Received: 20 April 2021
Commenter: Kai Aoki
Commenter's Conflict of Interests: Author
Comment: Changed the WB image to an accurate one.
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