Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Production of a β-Glucosidase-Rich Cocktail From Talaromyces Amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Wastes Valorization

Version 1 : Received: 14 April 2021 / Approved: 15 April 2021 / Online: 15 April 2021 (13:16:44 CEST)

A peer-reviewed article of this Preprint also exists.

Méndez-Liter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martinez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi 2021, 7, 363. Méndez-Liter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martinez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi 2021, 7, 363.

Journal reference: J. Fungi 2021, 7, 363
DOI: 10.3390/jof7050363

Abstract

As β-glucosidases represent the major bottleneck for industrial degradation of plant biomass, great efforts are being devoted both to discover novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme cocktails rich in this activity. Approximately 11 U/mL β-glucosidase were detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis revealed BGL-3 as the most abundant protein and the main β-glucosidase. This enzyme crude was successfully used to supplement a basal commercial cellulolytic cocktail (Cellu-clast 1.5L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of circular economy

Subject Areas

Fungi; hydrolases; cellulases; biodiesel by-products; wastes

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