Méndez-Liter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martinez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi2021, 7, 363.
Méndez-Liter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martinez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi 2021, 7, 363.
Méndez-Liter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martinez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi2021, 7, 363.
Méndez-Liter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martinez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi 2021, 7, 363.
Abstract
As β-glucosidases represent the major bottleneck for industrial degradation of plant biomass, great efforts are being devoted both to discover novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme cocktails rich in this activity. Approximately 11 U/mL β-glucosidase were detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis revealed BGL-3 as the most abundant protein and the main β-glucosidase. This enzyme crude was successfully used to supplement a basal commercial cellulolytic cocktail (Cellu-clast 1.5L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of circular economy
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
*
