Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of Magnaporthe oryzae

Version 1 : Received: 9 April 2021 / Approved: 12 April 2021 / Online: 12 April 2021 (12:34:48 CEST)

A peer-reviewed article of this Preprint also exists.

Bühring, B.; Yemelin, A.; Michna, T.; Tenzer, S.; Jacob, S. Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of Magnaporthe oryzae. J. Fungi 2021, 7, 389. Bühring, B.; Yemelin, A.; Michna, T.; Tenzer, S.; Jacob, S. Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of Magnaporthe oryzae. J. Fungi 2021, 7, 389.

Journal reference: J. Fungi 2021, 7, 389
DOI: 10.3390/jof7050389

Abstract

Different external stimuli are perceived by multiple sensor histidine kinases and transmitted by phosphorylation via the phosphotransfer protein Ypd1p in the multistep phosphorelay system of the high osmolarity glycerol signaling pathway of filamentous fungi. How the signal propagation takes place is still not known in detail, since multiple sensor histidine kinase genes in most filamentous fungi are coded in the genome, whereas only one gene for Ypd1 exists. That raises the hypothesis that various Ypd1 isoforms are produced from a single gene sequence, perhaps by alternative splicing, facilitating a higher variability in signal transduction. We found that the mRNA of MoYPD1 in the rice blast fungus Magnaporthe oryzae is subjected to an increased structural variation and amplified putative isoforms on a cDNA level. We then generated mutant strains overexpressing these isoforms, purified the products and present here one previously unknown MoYpd1 isoform on a proteome level. Alternative splicing was found to be a valid molecular mechanism to increase the signal diversity in eukaryotic multistep phosphorelay systems.

Keywords

phosphorelay; high osmolarity glycerol (HOG) pathway; alternative splicing; signaling; histidine kinases; Magnaporthe oryzae; YPD1; phosphotransfer; signal transduction

Subject

BIOLOGY, Anatomy & Morphology

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