Communication
Version 1
Preserved in Portico This version is not peer-reviewed
The Molecular Mechanism of Fludioxonil Action is Different to Osmotic Stress Sensing
Version 1
: Received: 9 April 2021 / Approved: 12 April 2021 / Online: 12 April 2021 (09:38:33 CEST)
A peer-reviewed article of this Preprint also exists.
Bersching, K.; Jacob, S. The Molecular Mechanism of Fludioxonil Action Is Different to Osmotic Stress Sensing. J. Fungi 2021, 7, 393. Bersching, K.; Jacob, S. The Molecular Mechanism of Fludioxonil Action Is Different to Osmotic Stress Sensing. J. Fungi 2021, 7, 393.
Abstract
The group III two-component hybrid histidine kinase MoHik1p in the filamentous fungus Magnaporthe oryzae is known to be a sensor for external osmotic stress and essential for the fungicidal activity of the phenylpyrrole fludioxonil. The mode of action of fludioxonil has not yet been completely clarified but rather assumed to hyperactivate the high osmolarity glycerol (HOG) signaling pathway. To date, not much is known about the detailed molecular mechanism of how osmotic stress is detected or fungicidal activity is initiated within the HOG pathway. The molecular mechanism of signaling was studied using a mutant strain in which the HisKA signaling domain was modified by an amino acid change of histidine H736 to alanine A736. We found that MoHik1pH736A is as resistant to fludioxonil but not as sensitive to osmotic stress as the null mutant ∆Mohik1. H736 is required for fludioxonil action but is not essential for sensing sorbitol stress. Consequently, this report provides evidence of the difference in the molecular mechanism of fludioxonil action and the perception of osmotic stress. This is an excellent basis to understand the successful phenylpyrrole-fungicides’ mode of action better and will give new ideas to decipher cellular signaling mechanisms.
Keywords
fludioxonil; fungicide; mode of action; high osmolarity glycerol (HOG) pathway; Magnaporthe oryzae; signal transduction; histidine kinase; MoHIK1; HIK1; phenylpyrrole
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments (0)
We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.
Leave a public commentSend a private comment to the author(s)
* All users must log in before leaving a comment
