Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Rapid and Sensitive Point of Care Detection of MRSA gDNA by Nanoelectrokinetic Sensors

Version 1 : Received: 27 March 2021 / Approved: 5 April 2021 / Online: 5 April 2021 (10:11:51 CEST)

A peer-reviewed article of this Preprint also exists.

Oueslati, R.; Jiang, Y.; Chen, J.; Wu, J. Rapid and Sensitive Point of Care Detection of MRSA Genomic DNA by Nanoelectrokinetic Sensors. Chemosensors 2021, 9, 97. Oueslati, R.; Jiang, Y.; Chen, J.; Wu, J. Rapid and Sensitive Point of Care Detection of MRSA Genomic DNA by Nanoelectrokinetic Sensors. Chemosensors 2021, 9, 97.

Journal reference: Chemosensors 2021, 9, 97
DOI: 10.3390/chemosensors9050097

Abstract

Biosensors have shown great potential in realizing rapid, low cost and portable on-site detection for diseases. This work reports the development of a new bioelectronic sensor called AC electrokinetics-based capacitive (ABC) biosensor, for the detection of genomic DNA (gDNA) of methicillin-resistant Staphylococcus aureus (MRSA). The ABC sensor is based on interdigitated microelectrodes biofunctionalized with oligonucleotide probes. It uses a special AC signal for direct capacitive monitoring of topological change on nanostructured sensor surface, which simultaneously induce dieletrophoretic enrichment of target gDNAs. As a result, rapid and specific detection of gDNA/probe hybridization can be realized with high sensitivity. It requires no signal amplification such as labelling, hybridization chain reaction, or nucleic acid sequence-based amplification. This method involves only simple sample preparation. After optimization of nano-structured sensor surface and signal processing, the ABC sensor demonstrated fast turnaround of results (~10 s detection), excellent sensitivity (a detection limit of 4.7 DNA copies /µL MRSA gDNA) and high specificity, suitable for point of care diagnosis. As a bioelectronic sensor, the developed ABC sensors can be easily adapted for detection of other infectious agents.

Keywords

DNA sensor; point of care diagnostics; methicillin-resistant Staphylococcus aureus; label free biosensor; AC electrokicetics

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