Oueslati, R.; Jiang, Y.; Chen, J.; Wu, J. Rapid and Sensitive Point of Care Detection of MRSA Genomic DNA by Nanoelectrokinetic Sensors. Chemosensors2021, 9, 97.
Oueslati, R.; Jiang, Y.; Chen, J.; Wu, J. Rapid and Sensitive Point of Care Detection of MRSA Genomic DNA by Nanoelectrokinetic Sensors. Chemosensors 2021, 9, 97.
Oueslati, R.; Jiang, Y.; Chen, J.; Wu, J. Rapid and Sensitive Point of Care Detection of MRSA Genomic DNA by Nanoelectrokinetic Sensors. Chemosensors2021, 9, 97.
Oueslati, R.; Jiang, Y.; Chen, J.; Wu, J. Rapid and Sensitive Point of Care Detection of MRSA Genomic DNA by Nanoelectrokinetic Sensors. Chemosensors 2021, 9, 97.
Abstract
Biosensors have shown great potential in realizing rapid, low cost and portable on-site detection for diseases. This work reports the development of a new bioelectronic sensor called AC electrokinetics-based capacitive (ABC) biosensor, for the detection of genomic DNA (gDNA) of methicillin-resistant Staphylococcus aureus (MRSA). The ABC sensor is based on interdigitated microelectrodes biofunctionalized with oligonucleotide probes. It uses a special AC signal for direct capacitive monitoring of topological change on nanostructured sensor surface, which simultaneously induce dieletrophoretic enrichment of target gDNAs. As a result, rapid and specific detection of gDNA/probe hybridization can be realized with high sensitivity. It requires no signal amplification such as labelling, hybridization chain reaction, or nucleic acid sequence-based amplification. This method involves only simple sample preparation. After optimization of nano-structured sensor surface and signal processing, the ABC sensor demonstrated fast turnaround of results (~10 s detection), excellent sensitivity (a detection limit of 4.7 DNA copies /µL MRSA gDNA) and high specificity, suitable for point of care diagnosis. As a bioelectronic sensor, the developed ABC sensors can be easily adapted for detection of other infectious agents.
Keywords
DNA sensor; point of care diagnostics; methicillin-resistant Staphylococcus aureus; label free biosensor; AC electrokicetics
Subject
Engineering, Electrical and Electronic Engineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
