Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Synthesis and evaluation of AlgNa-g-poly(QCL-co-HEMA) hydrogels for cartilage tissue engineering and controlled release of betamethasone.

Jomarien García-Couce
ORCID logo ,
Marioly Vernhes
,
Nancy Bada
,
Lissette Agüero
,
Oscar Valdés
ORCID logo ,
José Alvarez-Barreto
,
Gastón Fuentes
* ,
Amisel Almirall
ORCID logo ,
Luis J Cruz Ricondo
Version 1 : Received: 31 March 2021 / Approved: 2 April 2021 / Online: 2 April 2021 (11:20:34 CEST)
Version 2 : Received: 5 April 2021 / Approved: 6 April 2021 / Online: 6 April 2021 (10:03:04 CEST)

A peer-reviewed article of this Preprint also exists.

García-Couce, J.; Vernhes, M.; Bada, N.; Agüero, L.; Valdés, O.; Alvarez-Barreto, J.; Fuentes, G.; Almirall, A.; Cruz, L.J. Synthesis and Evaluation of AlgNa-g-poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone. Int. J. Mol. Sci. 2021, 22, 5730. García-Couce, J.; Vernhes, M.; Bada, N.; Agüero, L.; Valdés, O.; Alvarez-Barreto, J.; Fuentes, G.; Almirall, A.; Cruz, L.J. Synthesis and Evaluation of AlgNa-g-poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone. Int. J. Mol. Sci. 2021, 22, 5730.

Abstract

Hydrogels obtained from the combination of different polymers are an interesting strategy for the development of controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using different concentration of the components. The hydrogels were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and swelling degree; betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay shown that almost all hydrogels are cytocompatibles and an increase the proliferation in both cell types after one week of incubation was observed by Live/Dead® assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics and biocompatibility.

Keywords

Hydrogels; Sodium alginate; Betamethasone; Drug delivery; Cartilage tissue engineering

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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