Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Activity of Experimental Mouthwashes and Gels Containing DNA-RNA and Bioactive Molecules Against the Oxidative Stress of Oral Soft Tissues: The Importance of Formulations. a Bioreactor-Based Reconstituted Human Oral Epithelium Model

Version 1 : Received: 30 March 2021 / Approved: 31 March 2021 / Online: 31 March 2021 (17:45:14 CEST)

How to cite: Ionescu, A.C.; Vezzoli, E.; Conte, V.; Sartori, P.; Procacci, P.; Brambilla, E. Activity of Experimental Mouthwashes and Gels Containing DNA-RNA and Bioactive Molecules Against the Oxidative Stress of Oral Soft Tissues: The Importance of Formulations. a Bioreactor-Based Reconstituted Human Oral Epithelium Model. Preprints 2021, 2021030788 (doi: 10.20944/preprints202103.0788.v1). Ionescu, A.C.; Vezzoli, E.; Conte, V.; Sartori, P.; Procacci, P.; Brambilla, E. Activity of Experimental Mouthwashes and Gels Containing DNA-RNA and Bioactive Molecules Against the Oxidative Stress of Oral Soft Tissues: The Importance of Formulations. a Bioreactor-Based Reconstituted Human Oral Epithelium Model. Preprints 2021, 2021030788 (doi: 10.20944/preprints202103.0788.v1).

Abstract

Background: DNA-RNA compounds have shown promising protection against cell oxidative stress. This study aimed to assess the cytotoxicity, protective, or preventive effect of different experimental formulations on oral epithelia’s oxidative stress in vitro. Methods: Reconstituted human oral epithelia (RHOE) were grown air-lifted in a continuous-flow bioreactor. Mouthwash and gels containing DNA-RNA compounds and other bioactive molecules were tested on a model of oxidative stress generated by hydrogen peroxide treatment. Epithelia viability was evaluated using a biochemical MTT-based assay and confocal microscopy; structural and ultrastructural morphology was evaluated by light microscopy and TEM. Results: DNA-RNA showed non-cytotoxic activity and effectively protected against oxidative stress, but not in its prevention. Gel formulation did not express adequate activity compared to the mouthwash. Excipients played a fundamental role in enhancing or even decreasing the bioactive molecules’ effect. Conclusion: A mouthwash formulation with hydrolyzed DNA-RNA effectively protected against oxidative stress without additional enhancement by other bioactive molecules. Active compounds such as hyaluronic acid, β-Glucan, allantoin, bisabolol, ruscogenin, and essential oils showed a protective effect against oxidative stress, which was not synergistic with the one of DNA-RNA. Surfactant agents showed harmful activity against oral epithelia.

Subject Areas

DNA; RNA; bioactive compounds; excipients; surfactants; mouthwash; oral gels; oxidative stress; reconstituted oral epithelium; bioreactors

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