Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Statistical-Based Bioprocess Optimization of Amylase Production from Halophilic Bacillus sp. H7.

Version 1 : Received: 17 March 2021 / Approved: 18 March 2021 / Online: 18 March 2021 (12:22:42 CET)

A peer-reviewed article of this Preprint also exists.

Bandal, J.N.; Tile, V.A.; Sayyed, R.Z.; Jadhav, H.P.; Azelee, N.I.W.; Danish, S.; Datta, R. Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water. Molecules 2021, 26, 2833. Bandal, J.N.; Tile, V.A.; Sayyed, R.Z.; Jadhav, H.P.; Azelee, N.I.W.; Danish, S.; Datta, R. Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water. Molecules 2021, 26, 2833.

Journal reference: Molecules 2021, 26, 2833
DOI: 10.3390/molecules26102833

Abstract

Using the above results from RMS analysis the optimum values were predicted for the independent significant variables (Figure 3) the optimized levels of these variables in combination with other media variables the maximum production was predicted to be 199.90 U/mL. The predicted data were validated through confirmatory experiments performed in triplicates. A 1.29-fold increase in amylase activity against un-optimized (OVAT) medium was achieved in the present study authenticating the efficacy of RSM in process optimization (Figure 4). 2.6 Model validation and scale-up at laboratory scale (5L) bioreactor Once the parameters were standardized in the shake-flasks culture, the experiment was scaled-up to a laboratory-scale bioreactor (5 L). The yield of amylase increased by 1.01 fold (205.69 U/mL), it could be possible because the enzyme production in a bioreactor is higher than in shake-flasks culture as the various critical variable factors such as the dissolved oxygen (DO) and the pH can be optimally controlled at the desired levels [22].

Keywords

Amylase; Bacillus sp. H7; Optimization; Production; Response surface method

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.