Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chitosan from crabs (Scylla Serrata) Represses Hepato-renal Dysfunctions in Rats via Modulation of CD43 and p53 Expression in High Fat Diet-induced Hyperlipidemia

Version 1 : Received: 27 February 2021 / Approved: 2 March 2021 / Online: 2 March 2021 (16:02:07 CET)

A peer-reviewed article of this Preprint also exists.

Ugbaja, R.N.; Ogungbemi, K.; James, A.S.; Peter Folorunsho, A.; Abolade, S.O.; Ajamikoko, S.O.; Atayese, E.O.; Adedeji, O.V. Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats:Modulation of CD43 and p53 Expression. Pathophysiology 2021, 28, 224-237. Ugbaja, R.N.; Ogungbemi, K.; James, A.S.; Peter Folorunsho, A.; Abolade, S.O.; Ajamikoko, S.O.; Atayese, E.O.; Adedeji, O.V. Chitosan from Crabs (Scylla serrata) Represses Hyperlipidemia-Induced Hepato-Renal Dysfunctions in Rats:Modulation of CD43 and p53 Expression. Pathophysiology 2021, 28, 224-237.

Journal reference: Pathophysiology 2021, 28, 15
DOI: 10.3390/pathophysiology28020015

Abstract

Hepato-renal dysfunctions associated with hyperlipidemia necessitates continuous search for natural remedies. This study thus, evaluated the effect of dietary chitosan on diet-induced hyperlipidemic rats. Thirty male Wistar rats (90 ± 5.2) g were randomly allotted into six (6) groups (n=5): Normal diet, High-fat diet (HFD), Normal diet + 5% chitosan. The three other groups received HFD, supplemented with 1%-, 3%-, and 5% of chitosan. The feeding lasted for 8 weeks, after which the rats were sacrificed. The liver and kidneys were harvested for Analyses. Hepatic alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activity, and renal biomarkers (ALT, AST, urea, and creatinine) were assayed spectrophotometrically. Additionally, expression of hepatic and renal CD43 and p53 was estimated immunohistochemically. Hyperlipidemia caused a significant (p<0.05) decrease in the hepatic (AST, ALT, and ALP) and renal (AST and ALT) activities, while renal urea and creatinine increased. Furthermore, the HFD group showed an elevated level of hepatic and renal CD43 while p53 expression decreased. However, groups supplemented with chitosan showed improved hepatic and renal biomarkers, as well as corrected the aberrations in the expressions of p53 and CD43. Conclusively, dietary chitosan could effectively improve kidney and liver functionality via abatement of inflammatory responses.

Keywords

Chitosan; Hyperlipidemia; High-fat diet; p53; CD43

Subject

LIFE SCIENCES, Biochemistry

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