Version 1
: Received: 25 February 2021 / Approved: 1 March 2021 / Online: 1 March 2021 (14:19:14 CET)
How to cite:
Gutierrez, S. An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates. Preprints2021, 2021030030. https://doi.org/10.20944/preprints202103.0030.v1
Gutierrez, S. An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates. Preprints 2021, 2021030030. https://doi.org/10.20944/preprints202103.0030.v1
Gutierrez, S. An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates. Preprints2021, 2021030030. https://doi.org/10.20944/preprints202103.0030.v1
APA Style
Gutierrez, S. (2021). An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates. Preprints. https://doi.org/10.20944/preprints202103.0030.v1
Chicago/Turabian Style
Gutierrez, S. 2021 "An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates" Preprints. https://doi.org/10.20944/preprints202103.0030.v1
Abstract
Marine invertebrates are model organisms in several areas of biological sciences, being a source of massive biological information. Although, the scientific relevance of marine invertebrates, the research with them can be limited for their tissue characteristics and troubles for the replication of physical and chemical properties of seawater. Thence, the main goal of this laboratory workflow is to provide a useful methodological approach to reduce the experimental limitations during the study of marine invertebrates. The present studydescribes experimental methodologies for the collection, transport, and maintenance of sessile tunicates. Also, an approach to observe and characterize, a diverse population of blood cells in marine invertebrates, by several cytological stains and electron microscopy. Lastly, suggestions and protocols to extract quality RNA from samples with high concentrations of salts, pigments, secondary metabolites, and polysaccharides. This methodological approach can be easily adapted to other marine invertebrates, moreover uses low-cost reagents and widely available laboratory equipment. Making possible the study of different types of marine animals in diverse locations.
Keywords
Marine invertebrates; RNA extraction by LiCl precipitation; electron microscopy of blood cells; cytology stains; tunicate culture
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
