Preprint Technical Note Version 1 Preserved in Portico This version is not peer-reviewed

An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates

Version 1 : Received: 25 February 2021 / Approved: 1 March 2021 / Online: 1 March 2021 (14:19:14 CET)

How to cite: Gutierrez, S. An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates. Preprints 2021, 2021030030 (doi: 10.20944/preprints202103.0030.v1). Gutierrez, S. An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates. Preprints 2021, 2021030030 (doi: 10.20944/preprints202103.0030.v1).

Abstract

Marine invertebrates are model organisms in several areas of biological sciences, being a source of massive biological information. Although, the scientific relevance of marine invertebrates, the research with them can be limited for their tissue characteristics and troubles for the replication of physical and chemical properties of seawater. Thence, the main goal of this laboratory workflow is to provide a useful methodological approach to reduce the experimental limitations during the study of marine invertebrates. The present study describes experimental methodologies for the collection, transport, and maintenance of sessile tunicates. Also, an approach to observe and characterize, a diverse population of blood cells in marine invertebrates, by several cytological stains and electron microscopy. Lastly, suggestions and protocols to extract quality RNA from samples with high concentrations of salts, pigments, secondary metabolites, and polysaccharides. This methodological approach can be easily adapted to other marine invertebrates, moreover uses low-cost reagents and widely available laboratory equipment. Making possible the study of different types of marine animals in diverse locations.

Subject Areas

Marine invertebrates; RNA extraction by LiCl precipitation; electron microscopy of blood cells; cytology stains; tunicate culture

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.