Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established From Camelus Dromedaries Skeletal Muscle, Dermal Skin, and Adipose Tissues

Version 1 : Received: 25 February 2021 / Approved: 1 March 2021 / Online: 1 March 2021 (17:40:43 CET)

A peer-reviewed article of this Preprint also exists.

Son, Y.-B.; Jeong, Y.I.; Jeong, Y.W.; Hossein, M.S.; Tinson, A.; Singh, K.K.; Hwang, W.S. Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established from Camelus dromedarius Skeletal Muscle, Dermal Skin, and Adipose Tissues. Animals 2021, 11, 1017. Son, Y.-B.; Jeong, Y.I.; Jeong, Y.W.; Hossein, M.S.; Tinson, A.; Singh, K.K.; Hwang, W.S. Comparative Study of Biological Characteristics, and Osteoblast Differentiation of Mesenchymal Stem Cell Established from Camelus dromedarius Skeletal Muscle, Dermal Skin, and Adipose Tissues. Animals 2021, 11, 1017.

Journal reference: Animals 2021, 11, 1017
DOI: 10.3390/ani11041017

Abstract

Mesenchymal stem cells (MSCs) showed in vitro mesoderm-lineage differentiation and self-renew capacity. However, no comparative study was reported on the biological characteristics of stem cells derived from skeletal muscle (SM-MSCs), dermal skin (DS-MSCs), and adipose tissues (A-MSCs) from a single donor in camels. The present study aimed to evaluate the influence of MSCs source on stem cell characteristics. We evaluated proliferation capacity and mesoderm-lineage differentiation potential from SM-MSCs, DS-MSCs, and A-MSCs. They showed spindle-like morphology after homogenization. The proliferation ability was no significant difference in all groups. Furthermore, the portion of the cell cycle and expression of pluripotent markers (Oct4, Sox2, and Nanog) were similar in all cell lines at passage 3. The differentiation capacity of A-MSCs into adipocytes was significantly higher than that of SM-MSCs and DS-MSCs. However, the osteoblast differentiation capacity of A-MSCs was significantly lower than that of SM-MSCs and DS-MSCs. Additionally, after osteoblast differentiation, the ALP activity and calcium content was significantly decreased in A-MSCs as compared to SM-MSCs and DS-MSCs. To the best of our knowledge, we primally established MSCs from the single camel and demonstrated their comparative characteristics including expression of pluripotent factors and proliferation, and in vitro differentiation capacity into adipocytes and osteoblasts.

Subject Areas

Mesenchymal stem cells; Camelus dromedaries; skeletal muscle; dermal skin; adipose tissue;differentiation

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.