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AlphaLogD Determination: A New Generation Reversed-Phase HPLC Method to Measure Lipophilicity on Neutral and Basic Small and Beyond-Rule-of-Five Compounds
Version 1
: Received: 18 February 2021 / Approved: 23 February 2021 / Online: 23 February 2021 (14:07:42 CET)
How to cite:
Katz, D.; Fike, K.; Longenberger, J.; Placko, S.; Philippe-Venec, L.; Chervenak, A. AlphaLogD Determination: A New Generation Reversed-Phase HPLC Method to Measure Lipophilicity on Neutral and Basic Small and Beyond-Rule-of-Five Compounds. Preprints2021, 2021020514. https://doi.org/10.20944/preprints202102.0514.v1
Katz, D.; Fike, K.; Longenberger, J.; Placko, S.; Philippe-Venec, L.; Chervenak, A. AlphaLogD Determination: A New Generation Reversed-Phase HPLC Method to Measure Lipophilicity on Neutral and Basic Small and Beyond-Rule-of-Five Compounds. Preprints 2021, 2021020514. https://doi.org/10.20944/preprints202102.0514.v1
Katz, D.; Fike, K.; Longenberger, J.; Placko, S.; Philippe-Venec, L.; Chervenak, A. AlphaLogD Determination: A New Generation Reversed-Phase HPLC Method to Measure Lipophilicity on Neutral and Basic Small and Beyond-Rule-of-Five Compounds. Preprints2021, 2021020514. https://doi.org/10.20944/preprints202102.0514.v1
APA Style
Katz, D., Fike, K., Longenberger, J., Placko, S., Philippe-Venec, L., & Chervenak, A. (2021). AlphaLogD Determination: A New Generation Reversed-Phase HPLC Method to Measure Lipophilicity on Neutral and Basic Small and Beyond-Rule-of-Five Compounds. Preprints. https://doi.org/10.20944/preprints202102.0514.v1
Chicago/Turabian Style
Katz, D., Laurence Philippe-Venec and Andrew Chervenak. 2021 "AlphaLogD Determination: A New Generation Reversed-Phase HPLC Method to Measure Lipophilicity on Neutral and Basic Small and Beyond-Rule-of-Five Compounds" Preprints. https://doi.org/10.20944/preprints202102.0514.v1
Abstract
Lipophilicity can be measured with different methods, such as Shake-Flask or liquid chromatography. HPLC presents the advantage of overcoming solubility issues and therefore extending the range of lipophilicity to high values. A specific HPLC method, called ELogD, had been developed 20 years ago on a C16-amide stationary phase, enhancing hydrophobic and hydrogen bond interactions to mimic octanol-water partition. The emergence of novel stationary phases added to the need for a less complex mobile phase has led to the development of a new HPLC assay called alphaLogD, applicable to neutral and basic compounds at pH 7.4, that combines superficially porous particles, a high number of equilibriums between solutes and stationary phase, and results in a lower number of isocratic methods to determine the logk’w at a higher throughput. Statistical studies have been run to successfully evaluate the alphaLogD method compared to the Shake-Flask method and to allow this lipophilicity measurement into the so-called Beyond-Rule-of-5-molecules space.
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
