Version 1
: Received: 16 February 2021 / Approved: 18 February 2021 / Online: 18 February 2021 (10:46:00 CET)
Version 2
: Received: 18 February 2021 / Approved: 19 February 2021 / Online: 19 February 2021 (09:53:32 CET)
How to cite:
Abdelsattar, A.; Nofal, R.; Makky, S.; El-Sayed, A.; El-Shibiny, A. A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples. Preprints2021, 2021020410. https://doi.org/10.20944/preprints202102.0410.v2
Abdelsattar, A.; Nofal, R.; Makky, S.; El-Sayed, A.; El-Shibiny, A. A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples. Preprints 2021, 2021020410. https://doi.org/10.20944/preprints202102.0410.v2
Abdelsattar, A.; Nofal, R.; Makky, S.; El-Sayed, A.; El-Shibiny, A. A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples. Preprints2021, 2021020410. https://doi.org/10.20944/preprints202102.0410.v2
APA Style
Abdelsattar, A., Nofal, R., Makky, S., El-Sayed, A., & El-Shibiny, A. (2021). A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples. Preprints. https://doi.org/10.20944/preprints202102.0410.v2
Chicago/Turabian Style
Abdelsattar, A., Amera El-Sayed and Ayman El-Shibiny. 2021 "A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples" Preprints. https://doi.org/10.20944/preprints202102.0410.v2
Abstract
In the post antimicrobial era, increasing attention is paid towards using bacteriophage (phage in short) therapy to control antibiotic-resistant bacteria. The first step in phage therapy applications is isolating highly efficient lytic phages or phage cocktails from various sources. When a double-layer- agar with around 0.7% agar in top agar is employed, it results in a low number of phage isolation with a poor resolution, and in many cases, you miss the phage. To address this problem, a low concentration of agar in top agar is examined for better phage isolation. Here, our results proved the efficiency of isolating phage upon formulating a double-layer agar with 0.3% agar in top agar. A sewage sample was collected then phages were isolated, purified, and spotted on a top layer agar with 0.3% agar. The results showed the possibility of isolating a higher number of phages on 0.3% top agar than 0.7%. The finding advocates using 0.3% top agar for the double-layer agar, as it will provide fast, better, and easy phage screening and isolation.
Keywords
Phage Purification; phage isolation; phage characterization; jumbo bacteriophages; top agar; double agar overlay; high throughput sequencing
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
19 February 2021
Commenter:
Abdallah Abdelsattar
Commenter's Conflict of Interests:
Author
Comment:
Thank you. I would like to announce that authors in the middle that are listed in the PDF are not included in the in citation as authors . I would like to add them with the same order without any change in PDF itself.
Commenter: Abdallah Abdelsattar
Commenter's Conflict of Interests: Author