Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Endothelial Autocrine Signaling through 2CXCL12/CXCR4/FoxM1 Axis Contributes to Severe Pulmonary Arterial Hypertension

Version 1 : Received: 12 February 2021 / Approved: 16 February 2021 / Online: 16 February 2021 (13:43:56 CET)
Version 2 : Received: 15 March 2021 / Approved: 15 March 2021 / Online: 15 March 2021 (13:09:42 CET)
Version 3 : Received: 20 March 2021 / Approved: 22 March 2021 / Online: 22 March 2021 (15:51:47 CET)

A peer-reviewed article of this Preprint also exists.

Yi, D.; Liu, B.; Wang, T.; Liao, Q.; Zhu, M.M.; Zhao, Y.-Y.; Dai, Z. Endothelial Autocrine Signaling through CXCL12/CXCR4/FoxM1 Axis Contributes to Severe Pulmonary Arterial Hypertension. Int. J. Mol. Sci. 2021, 22, 3182. Yi, D.; Liu, B.; Wang, T.; Liao, Q.; Zhu, M.M.; Zhao, Y.-Y.; Dai, Z. Endothelial Autocrine Signaling through CXCL12/CXCR4/FoxM1 Axis Contributes to Severe Pulmonary Arterial Hypertension. Int. J. Mol. Sci. 2021, 22, 3182.

Journal reference: Int. J. Mol. Sci. 2021, 22, 3182
DOI: 10.3390/ijms22063182

Abstract

Endothelial autocrine signaling is essential to maintain vascular hemostasis. There is limited in-formation about the role of endothelial autocrine signaling in regulating severe pulmonary vas-cular remodeling during the onset of pulmonary arterial hypertension (PAH). In this study, we employed the first severe PAH mouse model, Egln1Tie2Cre (Tie2Cre-mediated disruption of Egln1) mice, to identify the novel autocrine signaling mediating the pulmonary vascular endothelial cells (PVECs) hyperproliferation and the pathogenesis of PAH. PVECs isolated from Egln1Tie2Cre lung expressed upregulation of many growth factors or angiocrine factors such as CXCL12, and exhib-ited hyperproliferative phenotype in coincident with upregulation of proliferation specific tran-scriptional factor FoxM1. Treatment of CXCL12 on PVECs increased FoxM1 expression, which was blocked by CXCL12 receptor CXCR4 antagonist AMD3100 in culture human PVECs. Endo-thelial specific deletion of Cxcl12 (Egln1/Cxcl12Tie2 Cre) or AMD3100 treatment in Egln1Tie2Cre mice downregulated FoxM1 expression in vivo. We then generated and characterized a novel mouse model with endothelial specific FoxM1 deletion in Egln1Tie2Cre mice (Egln1/Foxm1Tie2Cre), and found that endothelial FoxM1 deletion reduced pulmonary vascular remodeling and right ventricular systolic pressure. Together, our study identified a novel mechanism of endothelial autocrine sig-naling in regulating PVECs hyperproliferation and pulmonary vascular remodeling in PAH.

Subject Areas

Angiogenesis; Pulmonary hypertension; Endothelium; PHD2 Deficiency; Hypoxia

Comments (1)

Comment 1
Received: 15 March 2021
Commenter: Zhiyu Dai
Commenter's Conflict of Interests: Author
Comment: We have expanded the figure set and updated based on reviewers' suggestion.
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