Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Triterpenes and Phenolic Compounds From the Fungus Fuscoporia torulosa: Isolation, Structure Determination and Biological Activity

Version 1 : Received: 10 February 2021 / Approved: 11 February 2021 / Online: 11 February 2021 (11:45:31 CET)

How to cite: Béni, Z.; Dékány, M.; Sárközy, A.; Kincses, A.; Spengler, G.; Papp, V.; Hohmann, J.; Ványolós, A. Triterpenes and Phenolic Compounds From the Fungus Fuscoporia torulosa: Isolation, Structure Determination and Biological Activity. Preprints 2021, 2021020282 (doi: 10.20944/preprints202102.0282.v1). Béni, Z.; Dékány, M.; Sárközy, A.; Kincses, A.; Spengler, G.; Papp, V.; Hohmann, J.; Ványolós, A. Triterpenes and Phenolic Compounds From the Fungus Fuscoporia torulosa: Isolation, Structure Determination and Biological Activity. Preprints 2021, 2021020282 (doi: 10.20944/preprints202102.0282.v1).

Abstract

Investigation of the methanol extract of the poroid fungus Fuscoporia torulosa resulted in the isolation of a novel triterpene, fuscoporic acid (1) together with inoscavin A and its previously undescribed Z isomer (2 and 3), 3,4-dihydroxy-benzaldehide (4), osmundacetone (5), senexdiolic acid (6), natalic acid (7), and ergosta-7,22-diene-3-one (8). The structures of fungal compounds were determined on the basis of NMR and MS spectroscopic analysis, as well as molecular modelling studies. Compounds 1, 6-8 were examined for their antibacterial properties on resistant clinical isolates, and cytotoxic activity on human colon adenocarcinoma cell lines. Compound 8 was effective against Colo 205 (IC50 11.65±1.67 µM), Colo 320 (IC50 8.43±1.1 µM) and MRC-5 (IC50 7.92±1.42 µM) cell lines. Potentially synergistic relationship was investigated between 8 and doxorubicin, which revealed a synergism between the examined compounds with a combination index (CI) at the 50% growth inhibition dose (ED50) of 0.521±0.15. Several compounds (1, and 6-8) were tested for P‐glycoprotein modulatory effect in Colo 320 resistant cancer cells, but none of the compounds proved to be effective in this assay. Fungal metabolites 2-5 were evaluated for their antioxidant activity using the oxygen radical absorbance capacity (ORAC) and DPPH assays. Compounds 4 and 5 proved to possess considerable antioxidant effect with EC50 0.25±0.01 (DPPH) and 12.20±0.92 mmol TE/g (ORAC). The current article provides valuable information on both chemical and pharmacological profiles of Fuscoporia torulosa, paving the way for future studies with this species.

Subject Areas

Fuscoporia torulosa; triterpenes; cytotoxic; antioxidant; synergism; antibacterial; ORAC; DPPH

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