Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Identification of Cryptosporidium spp. in Patients Living with HIV / AIDS in Honduras

Version 1 : Received: 3 February 2021 / Approved: 4 February 2021 / Online: 4 February 2021 (14:42:28 CET)

A peer-reviewed article of this Preprint also exists.

Betancourth, S.; Archaga, O.; Moncada, W.; Rodríguez, V.; Fontecha, G. First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras. Pathogens 2021, 10, 336. Betancourth, S.; Archaga, O.; Moncada, W.; Rodríguez, V.; Fontecha, G. First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras. Pathogens 2021, 10, 336.

Journal reference: Pathogens 2021, 10, 336
DOI: 10.3390/pathogens10030336

Abstract

Cryptosporidiosis is one of the most important causes of gastroenteritis in the world, especially in low- and middle-income countries. It is caused by the Apicomplexan parasite Cryptosporidium spp., and mainly affects children and immunosuppressed people, in whom it can pose a serious risk to their health, or even be life-threatening. In Honduras there are no data on parasite species or on molecular diversity or Cryptosporidium subtypes. Therefore, a cross-sectional study was conducted between September 2019 and March 2020 for the molecular identification of Cryptosporidium spp. in 102 patients living with HIV who attended a national hospital in Tegucigalpa. Stool samples were analysed by direct microscopy, acid-fast stained smears, and a rapid lateral flow immunochromatographic test. All samples that tested positive were molecularly analyzed to identify the species and subtype of the parasite using three different markers: gp60, cowp, and 18Sr. PCR products were also sequenced. Four out of 102 samples (3.92%) were positive for Cryptosporidium parvum, and all were assigned to subtype IIa. These findings suggest a possible zoonotic transmission in this population.

Subject Areas

Cryptosporidium spp; C. parvum; gp60; 18S ribosomal; cowp; HIV / AIDS; Honduras

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