Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Copper Oxide Nanoparticles Impair Serum Biochemical Indices, Induce Histopathological Effects, and Modulate Transcription of Pro-Inflammatory Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus

Version 1 : Received: 24 January 2021 / Approved: 25 January 2021 / Online: 25 January 2021 (11:31:48 CET)

A peer-reviewed article of this Preprint also exists.

Abdel-Latif, H.M.; Dawood, M.A.; Mahmoud, S.F.; Shukry, M.; Noreldin, A.E.; Ghetas, H.A.; Khallaf, M.A. Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. Animals 2021, 11, 652. Abdel-Latif, H.M.; Dawood, M.A.; Mahmoud, S.F.; Shukry, M.; Noreldin, A.E.; Ghetas, H.A.; Khallaf, M.A. Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, HSP70, and Oxidative Stress Genes in Oreochromis niloticus. Animals 2021, 11, 652.

Journal reference: Animals 2021, 11, 652
DOI: 10.3390/ani11030652

Abstract

In the present study, fish were exposed to sub-lethal doses of CuONPs (68.92 ± 3.49 nm) (10, 20, and 50 mg/L) for a long exposure period (25 days). Compared to the control group (0.0 mg/L CuONPs), a significant dose-dependent elevation in blood urea and creatinine values, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase enzyme activities were evident in CuONPs-exposed groups (P < 0.05). Fish exposure to 50 mg/L CuONPs significantly upregulated the transcription of pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta, interleukin 12, and interleukin 8), heat shock protein 70, apoptosis-related gene (caspase 3), and oxidative stress-related (superoxide dismutase, catalase, and glutathione peroxidase) genes in liver and gills of the exposed fish in comparison with those in the control group (P < 0.05). Moreover, varying histopathological injuries were noticed in the hepatopancreatic tissues, posterior kidneys, and gills of fish groups correlated to the examined exposure dose of CuONPs. In summary, our results provide new insights and helpful information for better understanding the mechanisms of CuONPs toxicity in Nile tilapia at hematological, molecular levels, and tissue levels.

Subject Areas

Nile tilapia; CuONPs; Histopathology; Gene transcription; Toxicity

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