Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster

Version 1 : Received: 13 January 2021 / Approved: 14 January 2021 / Online: 14 January 2021 (08:07:44 CET)

How to cite: Proshkina, E.N.; Yushkova, E.; Koval, L.; Zemskaya, N.; Shchegoleva, E.; Solovev, I.; Yakovleva, D.; Minnikhanova, N.; Ulyasheva, N.; Shaposhnikov, M.; Moskalev, A. Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster. Preprints 2021, 2021010262 (doi: 10.20944/preprints202101.0262.v1). Proshkina, E.N.; Yushkova, E.; Koval, L.; Zemskaya, N.; Shchegoleva, E.; Solovev, I.; Yakovleva, D.; Minnikhanova, N.; Ulyasheva, N.; Shaposhnikov, M.; Moskalev, A. Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster. Preprints 2021, 2021010262 (doi: 10.20944/preprints202101.0262.v1).

Abstract

Small RNAs are essential for the coordination of many cellular processes, including the regulation of gene expression patterns, the prevention of genomic instability, and the suppression of mutagenic transposon activity. These processes determine aging, longevity, and sensitivity of cells and an organism to stress factors (particularly, ionizing radiation). The biogenesis and activity of small RNAs are provided by proteins of the Argonaute family. These proteins participate in the processing of small RNA precursors and the formation of an RNA-induced silencing complex. However, the role of Argonaute proteins in the regulation of lifespan and radioresistance remains poorly explored. We studied the effect of knockdown of Argonaute genes (AGO1, AGO2, AGO3, piwi) in various tissues on the Drosophila melanogaster lifespan and survival after the γ-irradiation at a dose of 700 Gy. In most cases, these parameters were reduced or did not change significantly in flies with tissue-specific RNA interference. Surprisingly, piwi knockdown in both the fat body and the nervous system caused a lifespan increase. But changes in radioresistance depended on the tissue in which the gene was knocked out. In addition, analysis of changes in retrotransposon levels and expression of stress response genes allowed us to determine associated molecular mechanisms.

Subject Areas

lifespan; aging; radioresistance; ionizing radiation; Agronaute; Piwi; Drosophila melanogaster

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