Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Can miRNA Indicate Risk of Illness After Continuous Exposure to M. tuberculosis?

Cleonardo Augusto Silva
,
Arthur Ribeiro-dos-Santos
,
Wanderson Gonçalves Gonçalves
ORCID logo ,
Pablo Pinto
,
Rafael Pompeu Pantoja
,
Tatiana Vinasco-Sandoval
ORCID logo ,
André Ribeiro-dos-Santos
,
Mara Helena Hutz
ORCID logo ,
Amanda Vidal
ORCID logo ,
Gilderlanio Santana Araújo
ORCID logo ,
Ândrea Ribeiro-dos-Santos
* ORCID logo ,
Sidney Santos
Version 1 : Received: 9 January 2021 / Approved: 11 January 2021 / Online: 11 January 2021 (12:59:32 CET)

A peer-reviewed article of this Preprint also exists.

Silva, C.A.; Ribeiro-dos-Santos, A.; Gonçalves, W.G.; Pinto, P.; Pantoja, R.P.; Vinasco-Sandoval, T.; Ribeiro-dos-Santos, A.M.; Hutz, M.H.; Vidal, A.F.; Araújo, G.S.; Ribeiro-dos-Santos, Â.; Santos, S. Can miRNA Indicate Risk of Illness after Continuous Exposure to M. tuberculosis? Int. J. Mol. Sci. 2021, 22, 3674. Silva, C.A.; Ribeiro-dos-Santos, A.; Gonçalves, W.G.; Pinto, P.; Pantoja, R.P.; Vinasco-Sandoval, T.; Ribeiro-dos-Santos, A.M.; Hutz, M.H.; Vidal, A.F.; Araújo, G.S.; Ribeiro-dos-Santos, Â.; Santos, S. Can miRNA Indicate Risk of Illness after Continuous Exposure to M. tuberculosis? Int. J. Mol. Sci. 2021, 22, 3674.

Abstract

Molecular studies regarding regulatory elements such as small ncRNAs and their mechanisms are poorly understood in infectious diseases. Tuberculosis is one of the oldest infectious diseases of humanity, and it is still a challenge to prevent and treat it. The control of the infection as well as its diagnosis are still complex, and treatments used are linked to several side effects. This study aimed to investigate miRNA’s expression profile to identify possible biomarkers for tuberculosis. We applied NGS techniques to investigate miRNA’s global expression profile from blood samples of infected patients with tuberculosis, their respective healthy physicians, and external healthy individuals as controls. Samples from 22 individuals run through a differential expression, target genes, gene set enrichment, and miRNA-gene network analysis. We observed 153 altered miRNAs, among which, only three DEmiRNAs (hsa-let-7g-5p, hsa-miR-486-3p and hsa-miR-4732-5p) were found between the investigated patients and their respective physicians. These DEmiRNAs are suggested to play an important role in granuloma regulation and their immune physiopathology. Our results propose that miRNAs may be involved in immune modulation, regulating the repertoire of genes expressed in the immune system’s cells. Our findings encourage the application of miRNAs as potential biomarkers for tuberculosis.

Keywords

miRNA; Tuberculosis; differential expression analysis

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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