Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform

Version 1 : Received: 5 January 2021 / Approved: 6 January 2021 / Online: 6 January 2021 (14:20:00 CET)

How to cite: Kang, D.H.; Kim, N.K.; Park, S.; Kang, H.W. VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform. Preprints 2021, 2021010119 (doi: 10.20944/preprints202101.0119.v1). Kang, D.H.; Kim, N.K.; Park, S.; Kang, H.W. VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform. Preprints 2021, 2021010119 (doi: 10.20944/preprints202101.0119.v1).

Abstract

Fluorescence-linked immunosorbent assay (FLISA) is a commonly used, quantitative technique for detecting biochemical based on antigen–antibody binding reactions using a well-plate platform. With the developments in the manufacturing technology of microfluidic systems, FLISA can be implemented onto microfluidic disk platforms, which allows the detection of trace biochemical with high resolutions. Apart from requiring a lower proportion of reagent (1/10), this method also reduces the time required for the entire process to less than an hour. The incubation process involves antigen–antibody binding reactions as well as the binding of fluorogenic substrates to target proteins. The protocol for FLISA on a microfluidic platform necessitates the appropriate execution of liquid reagent movements during each step in order to ensure sufficient binding reactions. Herein, we propose a novel microfluidic disk comprising a 3D incubation chamber. Vascular endothelial growth factor as concentration with ng mL-1 is detected sequentially using a benchtop process employing this 3D microfluidic disk. The 3D microfluidic disk is implemented without requiring manual intervention or additional procedures for liquid control. During the incubation process, microbead movement is controlled through centrifugal force, generated due to disk rotation, and gravitational force via bead sedimentation on the sloped floor of the chamber.

Subject Areas

Lab-on-a-disk; 3D microstructure; FLISA; VEGF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.