Version 1
: Received: 1 January 2021 / Approved: 5 January 2021 / Online: 5 January 2021 (10:04:48 CET)
How to cite:
Sersenová, D.; Machala, Z.; Repiská, V.; Gbelcová, H. The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells. Preprints2021, 2021010068. https://doi.org/10.20944/preprints202101.0068.v1
Sersenová, D.; Machala, Z.; Repiská, V.; Gbelcová, H. The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells. Preprints 2021, 2021010068. https://doi.org/10.20944/preprints202101.0068.v1
Sersenová, D.; Machala, Z.; Repiská, V.; Gbelcová, H. The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells. Preprints2021, 2021010068. https://doi.org/10.20944/preprints202101.0068.v1
APA Style
Sersenová, D., Machala, Z., Repiská, V., & Gbelcová, H. (2021). The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells. Preprints. https://doi.org/10.20944/preprints202101.0068.v1
Chicago/Turabian Style
Sersenová, D., Vanda Repiská and Helena Gbelcová. 2021 "The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells" Preprints. https://doi.org/10.20944/preprints202101.0068.v1
Abstract
Plasma medicine is a new field focusing on biomedical and clinical applications of cold physical plasmas, including their anticancer effects. Cold plasmas can be applied directly or indirectly as plasma activated liquids (PAL). The effect of plasma activated cell growth medium (PAM) and plasma activated phosphate buffered saline (PAPBS) were tested using a plasma pen generating streamer corona discharge in ambient air, on different cancer cell lines (melanoma A375, glioblastoma LN229 and pancreatic cancer MiaPaCa-2) and normal cells (human dermal fibroblasts HDFa). The viability reduction and apoptosis induction were detected in all cancer cells after incubation in PAL. In melanoma cells we focused on detailed insights to the apoptotic pathways. The anticancer effects depend on the plasma treatment time or PAL concentration. The first 30 minutes of incubation in PAL were enough to start processes leading to the cell death. In fibroblasts, no apoptosis induction was observed, only PAPBS, activated for longer time, slightly decreased their viability. Anticancer effects of PAM and PAPBS on cancer cells showed selectivity compared to normal fibroblasts, depended on correctly chosen activation time and PAL concentration. This selectivity, supported by optimum ratio of hydrogen peroxide and nitrites in PAL, is very promising for potential clinical applications.
Keywords
cold plasma; plasma activated liquid; cancer cell; melanoma; fibroblast
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
