Version 1
: Received: 21 December 2020 / Approved: 22 December 2020 / Online: 22 December 2020 (10:40:26 CET)
How to cite:
Pavlovska, I.; Ramata-Stunda, A.; Martinsone, Ž.; Boroduskis, M.; Patetko, L.; Martinsone, I.; Seile, A.; Vanadziņš, I. In Vitro Impact Preliminary Assessment of Airborne Particulate From Metalworking and Woodworking Industries. Preprints2020, 2020120552. https://doi.org/10.20944/preprints202012.0552.v1
Pavlovska, I.; Ramata-Stunda, A.; Martinsone, Ž.; Boroduskis, M.; Patetko, L.; Martinsone, I.; Seile, A.; Vanadziņš, I. In Vitro Impact Preliminary Assessment of Airborne Particulate From Metalworking and Woodworking Industries. Preprints 2020, 2020120552. https://doi.org/10.20944/preprints202012.0552.v1
Pavlovska, I.; Ramata-Stunda, A.; Martinsone, Ž.; Boroduskis, M.; Patetko, L.; Martinsone, I.; Seile, A.; Vanadziņš, I. In Vitro Impact Preliminary Assessment of Airborne Particulate From Metalworking and Woodworking Industries. Preprints2020, 2020120552. https://doi.org/10.20944/preprints202012.0552.v1
APA Style
Pavlovska, I., Ramata-Stunda, A., Martinsone, Ž., Boroduskis, M., Patetko, L., Martinsone, I., Seile, A., & Vanadziņš, I. (2020). In Vitro Impact Preliminary Assessment of Airborne Particulate From Metalworking and Woodworking Industries. Preprints. https://doi.org/10.20944/preprints202012.0552.v1
Chicago/Turabian Style
Pavlovska, I., Anita Seile and Ivars Vanadziņš. 2020 "In Vitro Impact Preliminary Assessment of Airborne Particulate From Metalworking and Woodworking Industries" Preprints. https://doi.org/10.20944/preprints202012.0552.v1
Abstract
Inhalation is the main route of exposure to airborne pollutants. To evaluate the safety and assess the risks of occupational hazards different testing approaches are used. 3D airway epithelial tissues allow to mimic exposure conditions in vitro, generates human-relevant toxicology data, allows to elucidate mode of action of pollutants. Gilian 3500 pumps equipped with Standard Midget Impingers were used to collect the airborne particulate from woodworking and metalworking environments. EpiAirway™ tissues were used to model half working day (4 h), full working day (8 h), and 3 working day exposures to occupational pollutants. Tissue viability was assessed using MTT assay. RT-qPCR analyses performed to analyze the expression of gelsolin, caspase-3, and IL-6. Tissue morphology was assessed by hematoxylin/eosin staining. Acute exposure to workspace pollutants slightly affected tissue viability and did not change the morphology. Both types of particles suppressed expression of gelsolin, with metalworking samples showing the most pronounced effect. A slight reduction in caspase-3 expression was observed. Particles from metalworking suppressed IL-6 expression. 3D Epithelial tissues can be used to model exposures to airborne pollutants. Exposure to particles from woodworking and metalworking had a minor effect on tissue viability but affected the expression of inflammation and apoptosis-related genes.
Keywords
Nanoparticles; metallic compounds; airborne particulate; occupational environment; in vitro exposure; alternative testing strategies; EpiAirway™; 3D tissue models; respiratory epithelial tissues
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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