Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Version 1 : Received: 10 December 2020 / Approved: 11 December 2020 / Online: 11 December 2020 (12:56:40 CET)

How to cite: Kelada, K.; Tusé, D.; Gleba, Y.; McDonald, K.; Nandi, S. Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II. Preprints 2020, 2020120280 (doi: 10.20944/preprints202012.0280.v1). Kelada, K.; Tusé, D.; Gleba, Y.; McDonald, K.; Nandi, S. Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II. Preprints 2020, 2020120280 (doi: 10.20944/preprints202012.0280.v1).

Abstract

There are currently worldwide efforts to reduce sugar intake due to the various adverse health effects linked with the overconsumption of sugars. Artificial sweeteners have been used as an alternative to nutritive sugars in numerous applications; however, their long-term effects on human health remain controversial. This led to a shift in consumer preference towards non-caloric sweeteners from natural sources. Thaumatins are a class of intensely sweet proteins found in arils of the fruits of the West-African plant Thaumatococcus danielli. Thaumatins’ current production method through aqueous extraction from this plant and uncertainty of the harvest from tropical rainforests limits its supply while the demand is increasing. Despite successful recombinant expression of the protein in several organisms, no large-scale bioproduction facilities exist. We present preliminary process design, process simulation, and economic analysis for a large-scale (50 metric tons/year) production of thaumatin II variant by several different molecular farming platforms.

Subject Areas

Thaumatin, sweet protein, molecular farming, natural sweeteners

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