Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Preparation and Characterization of a Bioartificial Polymeric System (Scaffold) Made of Chitosan and Polyvinyl Alcohol with Potential in the Viability of Bones Cells

Version 1 : Received: 3 December 2020 / Approved: 7 December 2020 / Online: 7 December 2020 (07:30:10 CET)

How to cite: Ramirez Tarazona, S.; Ochoa Melo, J.; Ángel García, J.; Bernal Ballén, A.; Segura Puello, H.R.; Villamil Ballesteros, A.; Nieto Mosquera, D.; Muñoz Forero, D. Preparation and Characterization of a Bioartificial Polymeric System (Scaffold) Made of Chitosan and Polyvinyl Alcohol with Potential in the Viability of Bones Cells. Preprints 2020, 2020120125 (doi: 10.20944/preprints202012.0125.v1). Ramirez Tarazona, S.; Ochoa Melo, J.; Ángel García, J.; Bernal Ballén, A.; Segura Puello, H.R.; Villamil Ballesteros, A.; Nieto Mosquera, D.; Muñoz Forero, D. Preparation and Characterization of a Bioartificial Polymeric System (Scaffold) Made of Chitosan and Polyvinyl Alcohol with Potential in the Viability of Bones Cells. Preprints 2020, 2020120125 (doi: 10.20944/preprints202012.0125.v1).

Abstract

Scaffolds are widely used in tissue engineering because their manufacture is based on natural and synthetic polymers, which allows them to have properties such as biocompatibility and biodegradability, creating an ideal environment for cell growth on their surface. In this context, among the polymers studied in Tissue Engineering are Chitosan (CH) and Polyvinyl Alcohol (PVA). CH is a versatile polymer obtained from de-acetylation of chitin, which is used for its high biodegradability and biocompatibility, although its mechanical properties must be improved. It has been found that one of the ways to improve the mechanical properties of CH is to mix it with other synthetic polymers such as PVA. PVA is known for its biocompatibility, biodegradability, zero toxicity and ease of preparation due to its solubility in water and excellent mechanical properties, such as tensile strength and ease in the formation of films and barriers. In this study we evaluated the capacity of scaffolds made with CH and PVA in different concentrations (2: 1, 1: 1, 1: 2, respectively) as a possible application in bone regeneration. This was made through different characterization tests such as Infrared Spectroscopy, AFM, Swelling test and Porosity test, where we obtained information about its structural and physicochemical properties. Additionally, a cellular quality control was performed on the material through the MTT assay. The Fourier transform infrared spectroscopy (FTIR) study showed that there are strong intermolecular hydrogen bonds between the chitosan and polyvinyl alcohol molecules. The Swelling and Porosity tests showed favorable results, obtaining maximum values ​​of 5519% and 72.17% respectively. MTT tests determined that the prepared materials are not cytotoxic. These findings suggest that scaffolds possess properties suitable for use in Tissue Engineering.

Subject Areas

biomaterials; cell control; chitosan; polyvinyl alcohol; scaffold; tissue engineering

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