Working Paper Article Version 1 This version is not peer-reviewed

Expression Pattern, Polymorphisms and Association Analyses of the Porcine NREP Gene

Version 1 : Received: 27 November 2020 / Approved: 1 December 2020 / Online: 1 December 2020 (08:48:46 CET)

How to cite: Knoll, A.; Nesvadbová, M.; Urban, T. Expression Pattern, Polymorphisms and Association Analyses of the Porcine NREP Gene. Preprints 2020, 2020120005 Knoll, A.; Nesvadbová, M.; Urban, T. Expression Pattern, Polymorphisms and Association Analyses of the Porcine NREP Gene. Preprints 2020, 2020120005

Abstract

The expression microarray technique was performed to investigate the differences in gene expression between Czech Large White pigs and wild boars in the longissimus lumborum et thoracis and biceps femoris muscle tissues. The NREP gene (neuronal regeneration related protein homolog) was selected for detailed study as an expressional and functional candidate gene. NREP plays a role in the transformation of neural, muscle and fibroblast cells and in smooth muscle myogenesis. Quantitative real-time PCR results confirmed that the porcine NREP gene was expressed in both skeletal muscles and significantly overexpressed in Czech Large White pigs compared to wild boars (P < 0.05). We identified 9 polymorphic sites in genomic DNA of NREP gene. Six of these polymorphisms were in complete linkage disequilibrium and therefore only 4 polymorphisms were informative. Associations of these 4 polymorphisms (HF571253:g.103G>A, HF571253:g.134G>A, HF571253:g.179T>C and HF571253:g.402_409delT) with meat performance traits were assessed in Czech Large White pigs. New polymorphisms in NREP gene were significantly associated with parameters of daily weight gain, lean meat and backfat thickness in Czech Large White pigs. Our primary study suggested that porcine NREP may play an important role in skeletal muscle growth, fat metabolism and meat performance traits.

Subject Areas

pig; NREP; gene expression; polymorphism; SNP; meat performance

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