Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Compounds with Anti-Onchocercal Activity from Voacanga africana Stapf (Apocynaceae): Identification and Molecular Modeling

Version 1 : Received: 16 November 2020 / Approved: 17 November 2020 / Online: 17 November 2020 (11:13:54 CET)

How to cite: Babiaka, S.; Simoben, C.; Abuga, K.; Mbah, J.; Karpoormath, R.; Ongarora, D.; Mugo, H.; Monya, E.; Cho-Ngwa, F.; Sippl, W.; Loveridge, J.; Ntie-Kang, F. Compounds with Anti-Onchocercal Activity from Voacanga africana Stapf (Apocynaceae): Identification and Molecular Modeling. Preprints 2020, 2020110446 (doi: 10.20944/preprints202011.0446.v1). Babiaka, S.; Simoben, C.; Abuga, K.; Mbah, J.; Karpoormath, R.; Ongarora, D.; Mugo, H.; Monya, E.; Cho-Ngwa, F.; Sippl, W.; Loveridge, J.; Ntie-Kang, F. Compounds with Anti-Onchocercal Activity from Voacanga africana Stapf (Apocynaceae): Identification and Molecular Modeling. Preprints 2020, 2020110446 (doi: 10.20944/preprints202011.0446.v1).

Abstract

A new iboga-vobasine-type isomeric bisindole alkaloid named voacamine A (1), along with eight known compounds, voacangine (2), voacristine (3), coronaridine (4), tabernanthine (5), iboxygaine (6), voacamine (7), voacorine (8), and conoduramine (9), were isolated from the stem bark of Voacanga africana. The structures of the compounds were determined by comprehensive spectroscopic analyses (1D- and 2D-NMR). Compounds 1, 2, 3, 4, 6, 7 and 8 were found to inhibit the motility of both the microfilariae (Mf) and adult male worms of Onchocerca ochengi, in a dose-dependent manner, but were only moderately active on the adult female worms upon biochemical assessment at 30 μM drug concentrations. The IC50 values of the isolates are 2.49-5.49 µM for microfilariae and 3.45-17.87 µM for adult males. Homology modeling was used to generate a 3D model of the the O. ochengi thioredoxin reductase target and docking simulation attempted to offer an explanation of the anti-onchocercal structure-activity relationship (SAR) of the isolated compounds. These alkaloids are new potential leads for the development of antifilirial drugs. The results of this study validate the traditional use of V. africana in the treatment of human onchocerciasis.

Subject Areas

Alkaloids; Anti-Onchocercal; Bisindoles; Docking; Homology Modeling; Monoindoles.

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