Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

The Generations of Sequencing Technologies: An Overview

Version 1 : Received: 10 November 2020 / Approved: 12 November 2020 / Online: 12 November 2020 (17:20:07 CET)

How to cite: Adesiyan, A.; Kade, E.; Ifeakachukwu, I.; Oladimeji, K.; Sowunmi, K.; Oladipupo, T. The Generations of Sequencing Technologies: An Overview. Preprints 2020, 2020110356 (doi: 10.20944/preprints202011.0356.v1). Adesiyan, A.; Kade, E.; Ifeakachukwu, I.; Oladimeji, K.; Sowunmi, K.; Oladipupo, T. The Generations of Sequencing Technologies: An Overview. Preprints 2020, 2020110356 (doi: 10.20944/preprints202011.0356.v1).

Abstract

The world has now entered into a replacement era of genomics due to the continued advancements within the next generation high throughput sequencing technologies, which incorporates sequencing by synthesis-fluorescent in place sequencing (FISSEQ), pyrosequencing, sequencing by ligation using polony amplification, supported oligonucleotide detection (SOLiD), sequencing by hybridization alongside sequencing by ligation, and nanopore technology. Great impacts of those methods are often seen for solving the genome related problems of plant and Animalia which will open the door of a replacement era of genomics. This might ultimately overcome the Sanger sequencing that ruled for 30 years. NGS is predicted to advance and make the drug discovery process more rapid.

Subject Areas

Sanger Sequencing; DNA Sequencing; Chain Termination Sequencing

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