Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Enhancing Mechanical Properties and Biological Performances of Injectable Bioactive Glass by Gelatin and Chitosan for Bone Small Defect Repair

Version 1 : Received: 4 November 2020 / Approved: 5 November 2020 / Online: 5 November 2020 (10:48:46 CET)

How to cite: Sohrabi, M.; Eftekhari Yekta, B.; Rezaie, H.; Naimi-Jamal, M.R.; Kumar, A.; Cochis, A.; Miola, M.; Rimondini, L. Enhancing Mechanical Properties and Biological Performances of Injectable Bioactive Glass by Gelatin and Chitosan for Bone Small Defect Repair. Preprints 2020, 2020110210 (doi: 10.20944/preprints202011.0210.v1). Sohrabi, M.; Eftekhari Yekta, B.; Rezaie, H.; Naimi-Jamal, M.R.; Kumar, A.; Cochis, A.; Miola, M.; Rimondini, L. Enhancing Mechanical Properties and Biological Performances of Injectable Bioactive Glass by Gelatin and Chitosan for Bone Small Defect Repair. Preprints 2020, 2020110210 (doi: 10.20944/preprints202011.0210.v1).

Abstract

Bioactive glass (BG) represents a promising biomaterial for bone healing; here injectable BG pastes biological properties were improved by 25 wt% gelatin or chitosan, as well as mechanical resistance was enhanced by adding 10 or 20 wt% 3-Glycidyloxypropyl trimethoxysilane (GPTMS) cross-linker. Composites exhibited bioactivity as apatite formation was observed by SEM and XRD after 14 days immersion in SBF; moreover, polymers did not enhance degradability as weight loss was >10% after 30 days in physiological conditions. BG-gelatin-20 wt% GPTMS composites demonstrated the highest compressive strength (4.8±0.5 MPa) in comparison with 100% BG control (1.9±0.1 MPa). Cytocompatibility was demonstrated towards human mesenchymal stem cells (hMSC), osteoblasts progenitors and endothelial cells. The presence of 20 wt% GPTMS conferred antibacterial properties thus inhibiting the joint pathogens Staphylococcus aureus and Staphylococcus epidermidis infection. Finally, hMSC osteogenesis was successfully supported in a 3D model as demonstrated by alkaline phosphatase release and osteogenic genes expression.

Subject Areas

bioactive glass; gelatin; chitosan; 3-Glycidyloxypropyl trimethoxysilane; bone.

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