Preprint Article Version 4 Preserved in Portico This version is not peer-reviewed

The Role of Divalent Transition Metal Ions in the Binding of Fur Dimer to DNA: Binding of Mn2+ and Co2+ to EC Fur dimer-DNA Complex

Version 1 : Received: 3 November 2020 / Approved: 3 November 2020 / Online: 3 November 2020 (12:49:52 CET)
Version 2 : Received: 3 November 2020 / Approved: 4 November 2020 / Online: 4 November 2020 (10:13:32 CET)
Version 3 : Received: 5 November 2020 / Approved: 6 November 2020 / Online: 6 November 2020 (08:51:39 CET)
Version 4 : Received: 9 November 2020 / Approved: 10 November 2020 / Online: 10 November 2020 (12:23:58 CET)
(This article belongs to the Research Topic Quantum Computing)

How to cite: Hamed, M.; Jabour, S. The Role of Divalent Transition Metal Ions in the Binding of Fur Dimer to DNA: Binding of Mn2+ and Co2+ to EC Fur dimer-DNA Complex. Preprints 2020, 2020110146 (doi: 10.20944/preprints202011.0146.v4). Hamed, M.; Jabour, S. The Role of Divalent Transition Metal Ions in the Binding of Fur Dimer to DNA: Binding of Mn2+ and Co2+ to EC Fur dimer-DNA Complex. Preprints 2020, 2020110146 (doi: 10.20944/preprints202011.0146.v4).

Abstract

Ferric uptake regulation protein is a repressor protein which binds an AT rich region of DNA (the iron box). Fur binds as a dimer in a helix turn helix mode and it is activated by iron(II) and other divalent transition metal ions at elevated concentrations in a process to regulate the ion uptake. Each transition metal ion induces certain conformational changes to aid the Fur binding, both the N-terminal and C-terminal domains take part in binding to DNA in addition to His 88 and His 86 residues. The process is discussed in view of experimental reports. Fe(II), Mn(II) and Co(II) activate Fur to bind DNA experimentally but Zinc plays a structural role and does not activate Fur to bind DNA.

Subject Areas

Ferric Uptake Regulation; Fur, Iron(II), Molecular dynamics; DNA; protein

Comments (1)

Comment 1
Received: 10 November 2020
Commenter: Mazen Hamed
Commenter's Conflict of Interests: Author
Comment: Figure added
text editted and improved, Abstract improved
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