Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Frugal Compound Microscopy for Microbiological Analysis and Education

Version 1 : Received: 29 October 2020 / Approved: 2 November 2020 / Online: 2 November 2020 (13:36:42 CET)

How to cite: anand, A. Frugal Compound Microscopy for Microbiological Analysis and Education. Preprints 2020, 2020110042. https://doi.org/10.20944/preprints202011.0042.v1 anand, A. Frugal Compound Microscopy for Microbiological Analysis and Education. Preprints 2020, 2020110042. https://doi.org/10.20944/preprints202011.0042.v1

Abstract

We report a very frugal microscope building method from easily realizable parts. We show a highly portable field ready compound type microscope with bright field, dark field and projection microscopy capabilities. It has good resolution and magnification for multiple aspects of education and diagnosis. We demonstrate that such a system can be built from simple lenses in laser pointers and camera modules with no specific 3d printed parts or costly lenses. We show all the parts of the system like stage, coarse and fine focussing system, microscope body, even slides can be built from commonly available materials like soda bottle and DVD disks. We list alternate, safer and easily available staining methods and chemicals. The microscope is developed with a target that it can be self-developed even in rural areas of the world with only resources at one’s disposal with very little education. This features a stable focussing and panning system for comfortable viewing through eyes and also easy imaging with a smartphone. We show its utility for microorganism analysis and potential for clinical diagnosis.

Keywords

compound microscope; inexpensive microscopy; ultra-low-cost microscope

Subject

Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics

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