Version 1
: Received: 20 October 2020 / Approved: 21 October 2020 / Online: 21 October 2020 (10:27:26 CEST)
How to cite:
Gencheva, D.; Beev, G. Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region. Preprints2020, 2020100426. https://doi.org/10.20944/preprints202010.0426.v1
Gencheva, D.; Beev, G. Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region. Preprints 2020, 2020100426. https://doi.org/10.20944/preprints202010.0426.v1
Gencheva, D.; Beev, G. Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region. Preprints2020, 2020100426. https://doi.org/10.20944/preprints202010.0426.v1
APA Style
Gencheva, D., & Beev, G. (2020). Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region. Preprints. https://doi.org/10.20944/preprints202010.0426.v1
Chicago/Turabian Style
Gencheva, D. and Georgi Beev. 2020 "Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region" Preprints. https://doi.org/10.20944/preprints202010.0426.v1
Abstract
Molecular identification via Internal Transcribed Spacer region (nrDNA-ITS) sequencing of Fusarium spp. isolates from wheat originated from Stara Zagora region, were performed for the first time in Bulgaria. А total of 60 wheat samples (Triticum aestivum) were morphologically identified at the genus level as Fusarium spp. in advance. The rDNA-ITS region of all isolates was successfully amplified and the PCR products obtained were directly sequenced. After a comparison of detected sequences with NCBI database, members of three different fungal genera (Fusarium, Chaetomium, and Alternaria) were identified. Among Fusarium isolates, the F. tricinctum was prevailing, followed by F. poae. A total of three isolate F. proliferatum, F. graminearum and F. equiseti were presented with a single probe. The lowest genetic distance (0.004) was detected between F. tricinctum isolates. On the base of genetic distances, fungal isolates were grouped in two main clusters – one comprising F. tricinctum isolates and F. proliferatum, and second including F. equiseti, F. graminearum and F. poae. It could be concluded that the rDNA-ITS genome region of the genus Fusarium may be used as a suitable marker of early detection, accurate and reliable identification of Fusarium spp. contamination of wheat. The timely and accurate information would assist in the selection of appropriate approaches for control of fusarium infections and possible mycotoxins contamination of agricultural production.
Keywords
Fusarium spp.; Triticum aestivum; Internal Transcribed Spacer region (rDNA-ITS); DNA sequencing
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
