Version 1
: Received: 10 October 2020 / Approved: 14 October 2020 / Online: 14 October 2020 (13:10:40 CEST)
Version 2
: Received: 28 May 2021 / Approved: 28 May 2021 / Online: 28 May 2021 (11:54:51 CEST)
Version 3
: Received: 17 June 2021 / Approved: 23 June 2021 / Online: 23 June 2021 (11:51:08 CEST)
How to cite:
Wang, M. Exogenous H2S Regulates of Cystathionine Gamma-Lyase in HUVECs During Hypoxia. Preprints2020, 2020100307. https://doi.org/10.20944/preprints202010.0307.v1
Wang, M. Exogenous H2S Regulates of Cystathionine Gamma-Lyase in HUVECs During Hypoxia. Preprints 2020, 2020100307. https://doi.org/10.20944/preprints202010.0307.v1
Wang, M. Exogenous H2S Regulates of Cystathionine Gamma-Lyase in HUVECs During Hypoxia. Preprints2020, 2020100307. https://doi.org/10.20944/preprints202010.0307.v1
APA Style
Wang, M. (2020). Exogenous H2S Regulates of Cystathionine Gamma-Lyase in HUVECs During Hypoxia. Preprints. https://doi.org/10.20944/preprints202010.0307.v1
Chicago/Turabian Style
Wang, M. 2020 "Exogenous H2S Regulates of Cystathionine Gamma-Lyase in HUVECs During Hypoxia" Preprints. https://doi.org/10.20944/preprints202010.0307.v1
Abstract
Cystathionine gamma-lyase (CSE) is one of the essential H2S-producing enzymes, and it regulates diverse functions in connection with cardiovascular function. It is crucial how exogenous H2S regulates CSE expression of the vascular endothelial cell during hypoxia. We examined the transcription and expression of CSE in HUVECs regulated by exogenous H2S with 100 μM during hypoxia by Luciferase assay, Western blotting, and quantitative RT-qPCR. Exogenous H2S influenced on the promoter activity of CSE in HUVECs during hypoxia. The effects of 100 μM H2S on CSE mRNA expression in HUVECs is decreased compared with 0 μM H2S. The consequences of 100 μM H2S on the expression level of CSE protein in HUVECs at two h of hypoxia is reduced compared with 0 μM H2S. These findings suggest that vascular endothelial cells can respond to the signals of hypoxia in the blood, and can respond to changes in H2S concentration in the blood, thus affect the blood vessels themselves.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.