Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fluorescence Immunoassays Using CdSe/ZnS Core/Shell Quantum Dots for the Determination of Progesterone in Human Serum

Version 1 : Received: 13 October 2020 / Approved: 13 October 2020 / Online: 13 October 2020 (07:52:14 CEST)

How to cite: Duong, H.D.; Oh, S.; Rhee, J.I. Fluorescence Immunoassays Using CdSe/ZnS Core/Shell Quantum Dots for the Determination of Progesterone in Human Serum. Preprints 2020, 2020100261 (doi: 10.20944/preprints202010.0261.v1). Duong, H.D.; Oh, S.; Rhee, J.I. Fluorescence Immunoassays Using CdSe/ZnS Core/Shell Quantum Dots for the Determination of Progesterone in Human Serum. Preprints 2020, 2020100261 (doi: 10.20944/preprints202010.0261.v1).

Abstract

In this study, two heterogeneous fluorescence immunoassays using CdSe/ZnS quantum dot (QD) to label anti-progesterone antibody (P4Ab) for the determination of progesterone (P4) were performed in the wells of a 96-well microtiter plate. First, P4Ab was conjugated to hydrophilic CdSe/ZnS QDs via ethyl-3-(dimethylaminopropyl) carbodiimide(EDC)-N-hydroxysuccinimide) chemistry(NHS) (QDs-P4Ab conjugates). The QDs-P4Ab conjugate was employed as a second antibody in a sandwich assay, where the P4Ab was immobilized onto the 3-aminopropyltrimethoxysilane (APTMS) sol-gel membrane of the wells of a 96-well microtiter plate, and P4 was bound between the immobilized P4Ab and the QDs-P4Ab conjugate. In this assay, the fluorescence intensity of the QDs increased with increasing P4 concentrations. This assay had a detection limit of 553.9 pg/ml and a sensitivity of 18,251.96 pg/ml with a linear range of 2,184.6 – 117,082 pg/ml. In the direct binding assay, P4 was directly bound to the QDs-P4Ab conjugates immobilized onto the APTMS sol-gel membrane of the wells of a 96-well microtiter plate. In this direct binding assay the fluorescence intensity of the QDs decreased with increasing P4 concentrations, and this assay had a linear range of 28.95 – 26,607.7 pg/ml with a detection limit of 3.32 pg/ml and a sensitivity of 987.24 pg/ml. These fluorescence immunoassays have been successfully applied for the determination of P4 in real human serum, and the results were well correlated with those of a certified radioimmunoassay (RIA) method.

Subject Areas

Fluorescence immunoassay; sol-gel; 96-well microtiter plate; CdSe/ZnS quantum dots (QDs); progesterone

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