Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Tetramethylbenzidine: A Photoacoustic Probe for Reactive Oxygen Species Detection

Roger Bresolí-Obach
ORCID logo ,
Marcello Frattini
,
Stefania Abbruzzetti
ORCID logo ,
Cristiano Viappiani
,
Montserrat Agut
ORCID logo ,
Santi Nonell
* ORCID logo
Version 1 : Received: 22 September 2020 / Approved: 23 September 2020 / Online: 23 September 2020 (04:17:34 CEST)

A peer-reviewed article of this Preprint also exists.

Bresolí-Obach, R.; Frattini, M.; Abbruzzetti, S.; Viappiani, C.; Agut, M.; Nonell, S. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors 2020, 20, 5952. Bresolí-Obach, R.; Frattini, M.; Abbruzzetti, S.; Viappiani, C.; Agut, M.; Nonell, S. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors 2020, 20, 5952.

Abstract

Photoacoustic imaging is attracting a great deal of interest owing to its distinct advantages over other imaging techniques such as fluorescence or magnetic resonance image. Availability of photoacoustic probes for reactive oxygen and nitrogen species (ROS/RNS) could shed light on a plethora of biological processes mediated by these key intermediates. Tetramethylbenzidine (TMB) is a non-toxic and non-mutagenic colorless dye that develops a distinctive blue color upon oxidation. In this work we have investigated the potential of TMB as photoacoustic probe for ROS/RNS. Our results indicate that TMB reacts with hypochlorite, hydrogen peroxide, singlet oxygen and nitrogen dioxide to produce the blue oxidation product, while ROS such as the superoxide radical anion, sodium peroxide, hydroxyl radical or peroxynitrite yield a colorless oxidation product. TMB does not penetrate Escherichia coli cytoplasm but is capable of detecting singlet oxygen generated in its outer membrane.

Keywords

3,3’,5,5’-tetramethylbenzidine; reactive oxygen species (ROS); reactive nitrogen species (RNS); photoacoustic probes; optical sensors; singlet oxygen

Subject

Chemistry and Materials Science, Physical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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