Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Duckweed, Lemna minor Modulates Heavy Metal-induced Oxidative Stress in Nile Tilapia

Version 1 : Received: 18 September 2020 / Approved: 20 September 2020 / Online: 20 September 2020 (14:41:41 CEST)

How to cite: Abdel-Gawad, F.K.; Khalil, W.K.; Bassem, S.M.; Kumar, V.; Parisi, C.; Inglese, S.; Temraz, T.A.; Nassar, H.F.; Guerriero, G. The Duckweed, Lemna minor Modulates Heavy Metal-induced Oxidative Stress in Nile Tilapia. Preprints 2020, 2020090475 (doi: 10.20944/preprints202009.0475.v1). Abdel-Gawad, F.K.; Khalil, W.K.; Bassem, S.M.; Kumar, V.; Parisi, C.; Inglese, S.; Temraz, T.A.; Nassar, H.F.; Guerriero, G. The Duckweed, Lemna minor Modulates Heavy Metal-induced Oxidative Stress in Nile Tilapia. Preprints 2020, 2020090475 (doi: 10.20944/preprints202009.0475.v1).

Abstract

A two-fold integrated research study was conducted; firstly, to understand effects of copper (Cu) and zinc (Zn) on the growth and oxidative stress in Nile tilapia, Oreochromis niloticus; secondly, to study the beneficial effects of the duckweed Lemna minor L. as a heavy metal remover from wastewater. Experiments were conducted in mesocosms with and without duckweed. Tilapia fingerlings were exposed to Cu (0.004 and 0.02 mg/L) and Zn (0.5 and 1.5 mg/L) and fish fed for four weeks. We evaluated the fish growth performance, the hepatic DNA structure using comet assay, the expression of antioxidative genes (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx and glutathione-S-transferase, GST) and GPx and GST enzymatic activity. The results showed that Zn exhibited more pronounced toxic effects than Cu. Low dose of Cu did not influence the growth whereas higher doses of Cu and Zn significantly reduced the growth rate of tilapia compared to control, but addition of duckweed prevented weight loss. Further, in the presence of a high dose of Cu and Zn, DNA damage decreased, antioxidant gene expressions and enzymatic activities increased. In conclusion, results suggest that duckweed and Nile tilapia can be suitable candidates in metal remediation wastewater assessment programs.

Subject Areas

Nile tilapia; Oreochromis niloticus; liver; duckweed; Lemna minor; Cu; Zn; Glutathione Peroxidase; GPx; Glutathione-S-Transferase; GST; Superoxide dismutase; SOD; Catalase; CAT; remediation assessment

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