Version 1
: Received: 18 September 2020 / Approved: 19 September 2020 / Online: 19 September 2020 (11:34:12 CEST)
How to cite:
Chaparala, S.; Iwema, C.L.; Chattopadhyay, A. SARS-CoV-2 Infections - Gene Expression Omnibus (GEO) Data Mining, Pathway Enrichment Analysis, and Prediction of Repurposable Drugs/Compounds. Preprints2020, 2020090459. https://doi.org/10.20944/preprints202009.0459.v1
Chaparala, S.; Iwema, C.L.; Chattopadhyay, A. SARS-CoV-2 Infections - Gene Expression Omnibus (GEO) Data Mining, Pathway Enrichment Analysis, and Prediction of Repurposable Drugs/Compounds. Preprints 2020, 2020090459. https://doi.org/10.20944/preprints202009.0459.v1
Chaparala, S.; Iwema, C.L.; Chattopadhyay, A. SARS-CoV-2 Infections - Gene Expression Omnibus (GEO) Data Mining, Pathway Enrichment Analysis, and Prediction of Repurposable Drugs/Compounds. Preprints2020, 2020090459. https://doi.org/10.20944/preprints202009.0459.v1
APA Style
Chaparala, S., Iwema, C.L., & Chattopadhyay, A. (2020). SARS-CoV-2 Infections - Gene Expression Omnibus (GEO) Data Mining, Pathway Enrichment Analysis, and Prediction of Repurposable Drugs/Compounds. Preprints. https://doi.org/10.20944/preprints202009.0459.v1
Chicago/Turabian Style
Chaparala, S., Carrie L Iwema and Ansuman Chattopadhyay. 2020 "SARS-CoV-2 Infections - Gene Expression Omnibus (GEO) Data Mining, Pathway Enrichment Analysis, and Prediction of Repurposable Drugs/Compounds" Preprints. https://doi.org/10.20944/preprints202009.0459.v1
Abstract
The COVID-19 global pandemic has created dire consequences with an alarming rate of morbidity and mortality. There are not yet vaccine or efficacious treatment options to combat the causative SARS-CoV-2 infection. This paper describes the identification of potentially repurposable drugs for COVID-19 treatment by conducting pathway enrichment analysis on publicly available Gene Expression Omnibus datasets. We first determined SARS-CoV-2 infection-induced alterations of host gene expressions and pathways. We then identified drugs or compounds that target and counter virus-triggered cellular perturbations, suggesting their potential repurposing for COVID-19 treatment. The key findings are that SARS-CoV-2 infection in host cells induces mitochondrial dysfunction, inhibits oxidative phosphorylation, and activates several immune response and pro-inflammatory pathways. Triptolide, the major bioactive component of a traditional Chinese medicine herb, may rescue mitochondrial dysfunction by activating oxidative phosphorylation. Further in vitro and in vivo studies are necessary to verify these results prior to clinical application.
COVID-19; coronavirus; drug repositioning; pathway enrichment
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
