Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Gnotobiotic Evaluation of Dalbergia sissoo Genotypes for Resistance against Fusarium solani via Dual Culture Set up

Version 1 : Received: 30 August 2020 / Approved: 31 August 2020 / Online: 31 August 2020 (16:21:02 CEST)

A peer-reviewed article of this Preprint also exists.

Chauhan, M., Thakur, A., Singh, Y. et al. Gnotobiotic evaluation of Dalbergia sissoo genotypes for resistance against Fusarium solani via dual culture set up. J Genet Eng Biotechnol 19, 35 (2021). https://doi.org/10.1186/s43141-021-00132-3 Chauhan, M., Thakur, A., Singh, Y. et al. Gnotobiotic evaluation of Dalbergia sissoo genotypes for resistance against Fusarium solani via dual culture set up. J Genet Eng Biotechnol 19, 35 (2021). https://doi.org/10.1186/s43141-021-00132-3

Abstract

Dalbergia sissoo (shisham), an important timber yielding multipurpose tree species of the Indian subcontinent, has been afflicted with large scale mortality due to wilt in natural forests and plantations, causing huge economic losses. Fusarium solani f. sp. dalbergiae (Fsd) has been identified as one of the causal organisms for wilt disease in D. sissoo. Present study comprises in vitro screening of ten selected genotypes of D. sissoo against two strains of Fsd in a dual culture set up under axenic condition. Callus and plantlets of ten genotypes of host plant were multiplied in vitro and were inoculated with conidial suspension of two strains of Fsd at three concentrations; 1× 101, 1× 103, and 1× 105 conidia/ml. Gnotobiotic evaluation of dual culture set up shows variations among D. sissoo genotypes in their response towards in vitro Fsd infection; and two genotypes (14 and 66) exhibited resistance against the pathogen strains. Callus of genotypes 14 and 66 significantly restricted the fungal mycelium growth whereas callus of remaining eight genotypes were completely infested by Fsd mycelium within 9 days. Similarly, plantlets of genotype 14 and 66, had lesser disease severity and remained green, and had fewer necrotic lesions in the roots whereas plantlets of remaining eight genotypes died within 15 days.

Keywords

Shisham mortality; Fusarium solani f. sp. dalbergiae; in vitro screening; genetic variation; Fusarium wilt; plant-microbe interaction; gnotobiota

Subject

Biology and Life Sciences, Forestry

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