Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Exploring the Regulatory Potential of Long Non-Coding RNA in Feed Efficiency of Indicine Cattle

Pamela A. Alexandre
* ORCID logo ,
Antonio Reverter
,
Laercio R. Porto-Neto
,
Roberta B. Berezin
,
Gabriela Ribeiro
,
Miguel Henrique Almeida Santana
,
José Bento Sterman Ferraz
,
Heidge Fukumasu
Version 1 : Received: 29 July 2020 / Approved: 30 July 2020 / Online: 30 July 2020 (09:39:36 CEST)

A peer-reviewed article of this Preprint also exists.

Alexandre, P.A.; Reverter, A.; Berezin, R.B.; Porto-Neto, L.R.; Ribeiro, G.; Santana, M.H.A.; Ferraz, J.B.S.; Fukumasu, H. Exploring the Regulatory Potential of Long Non-Coding RNA in Feed Efficiency of Indicine Cattle. Genes 2020, 11, 997. Alexandre, P.A.; Reverter, A.; Berezin, R.B.; Porto-Neto, L.R.; Ribeiro, G.; Santana, M.H.A.; Ferraz, J.B.S.; Fukumasu, H. Exploring the Regulatory Potential of Long Non-Coding RNA in Feed Efficiency of Indicine Cattle. Genes 2020, 11, 997.

Abstract

Long non-coding RNA (lncRNA) can regulate several aspects of gene expression, being associated with complex phenotypes in humans and livestock species. In taurine beef cattle, recent evidence points to the involvement of lncRNA in feed efficiency (FE), a proxy for increased productivity and sustainability. Here, we hypothesized specific regulatory roles of lncRNA in FE of indicine cattle. Using RNA-Seq data from liver, muscle, hypothalamus, pituitary and adrenal gland from Nellore bulls with divergent FE, we submitted new transcripts to a series of filters to confidently predict lncRNA. Then, we identified lncRNA that were differentially expressed (DE) and/or key regulators of FE. Finally, we explored lncRNA genomic location and interactions with miRNA and mRNA to infer potential function. We were able to identify 126 relevant lncRNA for FE in Bos indicus, some with high homology to previously identified lncRNA in Bos taurus and some possible specific regulators of FE in indicine cattle. Moreover, lncRNA identified here were linked to previously described mechanisms related to FE in hypothalamus-pituitary-adrenal axis and are expected to help elucidate this complex phenotype. This study contributes to expanding the catalogue of lncRNA, particularly in indicine cattle, and identifies candidates for further studies in animal selection and management.

Keywords

co-expression network; residual feed intake; RNA-Seq

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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