Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antibiotic Treatment in An. coluzzii Affects Carbon and Nitrogen Metabolism

Version 1 : Received: 15 July 2020 / Approved: 17 July 2020 / Online: 17 July 2020 (11:00:53 CEST)

A peer-reviewed article of this Preprint also exists.

Chabanol, E.; Behrends, V.; Prévot, G.; Christophides, G.K.; Gendrin, M. Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism. Pathogens 2020, 9, 679. Chabanol, E.; Behrends, V.; Prévot, G.; Christophides, G.K.; Gendrin, M. Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism. Pathogens 2020, 9, 679.

Abstract

The mosquito microbiota reduces the vector competence of Anopheles to Plasmodium and affects host fitness, it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota-Plasmodium interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of An. coluzzii provided with a sugar-meal or a blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified candidate pathways which may participate in microbiota/Plasmodium interactions via metabolic interactions or immune modulation.

Keywords

mosquito; Anopheles; microbiota; malaria; Plasmodium; metabolism; immunity; TCA cycle; nitrogen excretion; amino acids

Subject

Biology and Life Sciences, Insect Science

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