Preprint Article Version 1 This version is not peer-reviewed

The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil against Periodontal Bacteria and Candida sp. and Its Anti-inflammatory Potential

Version 1 : Received: 18 May 2020 / Approved: 19 May 2020 / Online: 19 May 2020 (03:45:56 CEST)

A peer-reviewed article of this Preprint also exists.

Milia, E.; Usai, M.; Szotáková, B.; Elstnerová, M.; Králová, V.; D’hallewin, G.; Spissu, Y.; Barberis, A.; Marchetti, M.; Bortone, A.; Campanella, V.; Mastandrea, G.; Langhansová, L.; Eick, S. The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential. Antibiotics 2020, 9, 281. Milia, E.; Usai, M.; Szotáková, B.; Elstnerová, M.; Králová, V.; D’hallewin, G.; Spissu, Y.; Barberis, A.; Marchetti, M.; Bortone, A.; Campanella, V.; Mastandrea, G.; Langhansová, L.; Eick, S. The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential. Antibiotics 2020, 9, 281.

Journal reference: Antibiotics 2020, 9, 281
DOI: 10.3390/antibiotics9060281

Abstract

Background: Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy), toward a wide range of periodontal bacteria and Candida including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety; Methods: PLL-EO was screened by gas chromatography / mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX 1/2) and lipoxygenase (LOX) inhibition while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four line of oral cells; Results: According to the concentrations of terpens, PLL-EO is a pharmacologically active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. were between 6.25 and 12.5 µg/ml. Oxidation by COX 1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose; Conclusions: PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX2 and LOX inflammatory enzymes and without side effects against oral cells.

Subject Areas

Oral health care products; cyclooxygenase; lipoxygenase; periodontal disease; Candida albicans; Candida glabrata; medicinal herbs

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