Honko, A.N.; Storm, N.; Bean, D.J.; Henao Vasquez, J.; Downs, S.N.; Griffiths, A. Rapid Quantification and Neutralization Assays for Novel Coronavirus SARS-CoV-2 Using Avicel RC-591 Semi-Solid Overlay. Preprints2020, 2020050264. https://doi.org/10.20944/preprints202005.0264.v1
Honko, A.N., Storm, N., Bean, D.J., Henao Vasquez, J., Downs, S.N., & Griffiths, A. (2020). Rapid Quantification and Neutralization Assays for Novel Coronavirus SARS-CoV-2 Using Avicel RC-591 Semi-Solid Overlay. Preprints. https://doi.org/10.20944/preprints202005.0264.v1
Honko, A.N., Sierra N. Downs and Anthony Griffiths. 2020 "Rapid Quantification and Neutralization Assays for Novel Coronavirus SARS-CoV-2 Using Avicel RC-591 Semi-Solid Overlay" Preprints. https://doi.org/10.20944/preprints202005.0264.v1
When working with the novel coronavirus SARS-CoV-2 during a pandemic response, having a rapid, reproducible and reliable assay for infectious virus quantitation and utilization for evaluation of potential therapeutics is critical. Compared to traditional agarose overlay plaques visualized with neutral red, assays performed with Avicel R RC-591 semi-solid overlay provide a simplified format for rapid and easy detection and neutralization testing. The method is easily modified for higher throughput using dispensers or automated processing. Fixation using formalin provides flexibility when dealing with pathogenic agents such as SARS-CoV-2 where tissue culture plates might be removed from biocontainment for staining. Although plaque assays are considered straightforward in principle, having an easily reproducible, consistent plaque assay is an invaluable tool.
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