Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Rapid Quantification and Neutralization Assays for Novel Coronavirus SARS-CoV-2 Using Avicel RC-591 Semi-Solid Overlay

Anna N. Honko
* ORCID logo ,
Nadia Storm
,
David J. Bean
,
Jhonatan Henao Vasquez
,
Sierra N. Downs
,
Anthony Griffiths
Version 1 : Received: 14 May 2020 / Approved: 16 May 2020 / Online: 16 May 2020 (15:51:52 CEST)

How to cite: Honko, A. N.; Storm, N.; Bean, D. J.; Henao Vasquez, J.; Downs, S. N.; Griffiths, A. Rapid Quantification and Neutralization Assays for Novel Coronavirus SARS-CoV-2 Using Avicel RC-591 Semi-Solid Overlay. Preprints 2020, 2020050264. https://doi.org/10.20944/preprints202005.0264.v1 Honko, A. N.; Storm, N.; Bean, D. J.; Henao Vasquez, J.; Downs, S. N.; Griffiths, A. Rapid Quantification and Neutralization Assays for Novel Coronavirus SARS-CoV-2 Using Avicel RC-591 Semi-Solid Overlay. Preprints 2020, 2020050264. https://doi.org/10.20944/preprints202005.0264.v1

Abstract

When working with the novel coronavirus SARS-CoV-2 during a pandemic response, having a rapid, reproducible and reliable assay for infectious virus quantitation and utilization for evaluation of potential therapeutics is critical. Compared to traditional agarose overlay plaques visualized with neutral red, assays performed with Avicel R RC-591 semi-solid overlay provide a simplified format for rapid and easy detection and neutralization testing. The method is easily modified for higher throughput using dispensers or automated processing. Fixation using formalin provides flexibility when dealing with pathogenic agents such as SARS-CoV-2 where tissue culture plates might be removed from biocontainment for staining. Although plaque assays are considered straightforward in principle, having an easily reproducible, consistent plaque assay is an invaluable tool.

Keywords

plaque assay; neutralization; SARS; SARS-CoV-2; coronavirus; Avicel; methylcellulose; COVID

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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